Efficacy of the entomopathogenic nematode isolate Heterorhabditis taysearae to control the cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae)

Abstract

Abstract Background Entomopathogenic nematodes (EPNs) are promising alternatives since they have many characteristics as a biological control agent against insect pests. Results Among indigenous EPN isolated, adapted to local environmental conditions by employing the Galleria baiting strategy, only one sample was positive for the presence of EPNs. The new isolate was identified at the species level using DNA sequencing of the internal transcribed spacer region and the Basic Local Alignment Search Tool search of GenBank showed that the isolate had a high similarity (99%) with that sequence available for Heterorhabditis taysearae. The pathogenicity of the EPN isolate was tested against the cotton leaf worm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae), using different concentrations (60, 90, 120 and 150 IJs/larvae). Data showed that 150 IJs/larvae caused 100% mortality rate, followed by 120 IJs/larvae (90%), while 60 IJs/larvae showed the lowest rate (60%) after 72 h. Conclusions The present study indicated that the native isolate of EPN could be recommended against S. littoralis as an efficient tool in its control programs.

Keywords

• Entomopathogenic nematodes
• Heterorhabditis taysearae
• ITS sequencing
• Spodoptera littoralis
• Potency