Communications Biology (Mar 2021)

TagBiFC technique allows long-term single-molecule tracking of protein-protein interactions in living cells

  • Shipeng Shao,
  • Hongchen Zhang,
  • Yong Zeng,
  • Yongliang Li,
  • Chaoying Sun,
  • Yujie Sun

DOI
https://doi.org/10.1038/s42003-021-01896-7
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 14

Abstract

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Shao et al. develop an imaging probe, TagBiFC, to visualize protein-protein interaction in living cells with better signal-to-noise ratio and photostability than fluorescent proteins. This probe provides better localization and longer tracking time and authors visualise transcription factor dimerization, single-molecule nucleosome, and background-free mRNA imaging using TagBiFC.