Determination of Antifungal Activity of Mentha spicata L. Essential Oils Against Different Isolates of Chickpea Blight Disease [Ascochyta rabiei (Pass) Labr.]

Abstract

This study was conducted to determine the antifungal activity of Mentha spicata L. essential oils against 5 different isolates of Chickpea Blight (Ascochyta rabiei) under in vitro conditions. The antifungal activity assay was performed in 60 mm Petri dishes containing 10 ml Potato-Dextrose-Agar (PDA) media. 5 mm diameter fungal discs were placed in the middle of Petri dishes. The essential oils was added to the sterile drying papers stuck to the Petri dishes using micropipette at doses of 0 (control), 1, 3, 5 and 10 μl. Petri dishes sealed with parafilm were incubated for 14 days at 25 ° C. Measurements were made after completion of the incubation period and percent inhibition of mycelial growth was determined. In conclusion, different doses of M. spicata essential oil inhibited fungus mycelial growth at different ratios, and 10 uL dose completely (100%) stopped mycelial growth of fungus. As a result of this study, it has been determined that M. spicata essential oils has a strong antifungal activity in vitro conditions.

Keywords

• Antracnose (Ascochyta rabiei)
• antifungal effect
• essential oil
• Mentha spicata L.