Neuroprotective effects of dendrobium endophytes metabolites in SH-SY5Y cells via the Nrf2/Keap1 pathway
Yan tian Liang,
Jia meng Liu,
Lu qi Qin,
Cong Lu,
Jing Sun,
Qiong Wang,
Yong Yang,
Bei Fan,
Feng zhong Wang
Affiliations
Yan tian Liang
College of Pharmacy, Hunan University of Traditional Chinese Medicine, Hunan, China
Jia meng Liu
Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China
Lu qi Qin
Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China
Cong Lu
Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China
Jing Sun
Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China
Qiong Wang
Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China
Yong Yang
College of Pharmacy, Hunan University of Traditional Chinese Medicine, Hunan, China
Bei Fan
Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China; Corresponding author.
Feng zhong Wang
Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China; Corresponding author.
Recent studies have revealed that endophytes in plants can produce metabolites with activity that is comparable to or identical to the host. Dendrobine has attracted much attention in the field of neurodegenerative diseases by exhibiting anti-oxidative stress and neuroprotective effects. This study aimed to investigate the protective effects and mechanisms of metabolites of dendrobium endophytes Pseudomonas protegens CM-YJ44 and Priestia megaterium D-HT207 against H2O2-induced oxidative stress injury in SH-SY5Y cells. Results showed that there were 50 neuroprotective compounds in CM-YJ44 and 72 neuroprotective compounds in D-HT207. Those both increased significantly cell viability, decreased contents of ROS in H2O2-induced SH-SY5Y cells. It was confirmed that metabolites of CM-YJ44 and D-HT207 inhibited the H2O2-induced oxidative stress injury in SH-SY5Y cells, which mechanism is related to inhibition of ROS production, alteration of MMP, and inhibition of apoptosis and inflammatory factors expression via the Nrf2/Keap1 pathway.
