Osteogenic differentiation of bone marrow stromal stem cells on a novel β titanium alloy-based micro-nano composite

Wenyue Yang; Xiao Liu; Yingjing Fang; Shokouh Attarilar; Chenyuan Zhu; Liqiang Wang; Qing Luo; Yuanfei Fu

Journal of Materials Research and Technology (May 2023)

Osteogenic differentiation of bone marrow stromal stem cells on a novel β titanium alloy-based micro-nano composite

Wenyue Yang,
Xiao Liu,
Yingjing Fang,
Shokouh Attarilar,
Chenyuan Zhu,
Liqiang Wang,
Qing Luo,
Yuanfei Fu

Affiliations

Wenyue Yang: Department of Prosthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, China
Xiao Liu: Department of Endodontics, Shanghai Xuhui District Dental Center, 500 Fenglin Road, Shanghai, 200031, China
Yingjing Fang: Department of Prosthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, China
Shokouh Attarilar: Department of Materials Engineering, Faculty of Engineering, University of Maragheh, Maragheh, Iran
Chenyuan Zhu: Department of Prosthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, China
Liqiang Wang: State Key Laboratory of Metal Matrix Composites, School of Material Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China; National Facility for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China; Corresponding author.
Qing Luo: Department of General Dentistry, Shanghai Xuhui District Dental Center, 500 Fenglin Road, Shanghai, 200031, China; Corresponding author.
Yuanfei Fu: Department of Prosthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, China; Department of Dental Technology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, China; Corresponding author.

Journal volume & issue: Vol. 24
pp. 5864 – 5875

Abstract

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The incorporation of nanoparticles into metallic biomaterials has been recognized as a significant approach to improve their bioactivity for biomedical applications. In this study, friction stir processing (FSP) was employed to incorporate TiO2 nanoparticles as bioactive reinforcement into the β titanium alloy Ti–35Nb–2Ta–3Zr (TNTZ) matrix to construct an integrated micro-nano composite layer. The effect of different TiO2 contents on the osteogenic capacity of rat bone marrow stromal stem cells (BMSCs) was studied. The TiO2/TNTZ composite layer exhibited a refined grain structure with a mean grain size of approximately 10 μm and a characteristic surface morphology of pronounced peaks and valleys with enhanced RSkw and RKur values. Moreover, TiO2/TNTZ significantly promoted the adhesion, proliferation, and spreading of BMSCs, upregulated the expression of genes related to osteogenesis (ALP and Runx2) up to nearly 1.5 times, and enhanced the expression of ALP and OCN (P < 0.05). The novel FSPed TiO2/TNTZ micro-nano composite possesses excellent surface characteristics and favorable biological activities, manifesting vast potential for upcoming clinical applications.

Published in Journal of Materials Research and Technology

ISSN: 2238-7854 (Print); 2214-0697 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Mining engineering. Metallurgy
Website: https://www.journals.elsevier.com/journal-of-materials-research-and-technology/

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