Impact of Drought and Flooding on Alkaloid Production in <i>Annona crassiflora</i> Mart
Ana Beatriz Marques Honório,
Iván De-la-Cruz-Chacón,
Mariano Martínez-Vázquez,
Magali Ribeiro da Silva,
Felipe Girotto Campos,
Bruna Cavinatti Martin,
Gustavo Cabral da Silva,
Carmen Sílvia Fernandes Boaro,
Gisela Ferreira
Affiliations
Ana Beatriz Marques Honório
Plant Biology Sector, Biostatistics, Plant Biology, Parasitology and Zoology Department, Institute of Biosciences, São Paulo State University, UNESP, Street Prof. Dr. Antônio Celso Wagner Zanin 250, Botucatu 18618-689, SP, Brazil
Iván De-la-Cruz-Chacón
Laboratorio de Fisiología y Química Vegetal, Instituto de Ciencias Biológicas, Universidad de Ciencias y Artes de Chiapas, UNICACH, Libramiento Norte Poniente 1150, Tuxtla Gutiérrez 29039, CHIS, Mexico
Mariano Martínez-Vázquez
Chemistry Institute, Universidad Nacional Autónoma de México, UNAM, Circuito Exterior S/N, C.U., Coyoacán 04510, CDMX, Mexico
Magali Ribeiro da Silva
Forest, Soil and Environmental Sciences Department, Faculty of Agronomic Science, São Paulo State University, UNESP, Avenida Universitária 3780, Botucatu 18610-034, SP, Brazil
Felipe Girotto Campos
Plant Biology Sector, Biostatistics, Plant Biology, Parasitology and Zoology Department, Institute of Biosciences, São Paulo State University, UNESP, Street Prof. Dr. Antônio Celso Wagner Zanin 250, Botucatu 18618-689, SP, Brazil
Bruna Cavinatti Martin
Plant Biology Sector, Biostatistics, Plant Biology, Parasitology and Zoology Department, Institute of Biosciences, São Paulo State University, UNESP, Street Prof. Dr. Antônio Celso Wagner Zanin 250, Botucatu 18618-689, SP, Brazil
Gustavo Cabral da Silva
Plant Biology Sector, Biostatistics, Plant Biology, Parasitology and Zoology Department, Institute of Biosciences, São Paulo State University, UNESP, Street Prof. Dr. Antônio Celso Wagner Zanin 250, Botucatu 18618-689, SP, Brazil
Carmen Sílvia Fernandes Boaro
Plant Biology Sector, Biostatistics, Plant Biology, Parasitology and Zoology Department, Institute of Biosciences, São Paulo State University, UNESP, Street Prof. Dr. Antônio Celso Wagner Zanin 250, Botucatu 18618-689, SP, Brazil
Gisela Ferreira
Plant Biology Sector, Biostatistics, Plant Biology, Parasitology and Zoology Department, Institute of Biosciences, São Paulo State University, UNESP, Street Prof. Dr. Antônio Celso Wagner Zanin 250, Botucatu 18618-689, SP, Brazil
The Brazilian Cerrado is the second largest Brazilian biome. In recent decades, a reduction in rainfall has indicated an extension of the dry season. Among the many native species of the Cerrado of the Annonaceae family and used in folk medicine, Annona crassiflora Mart. has fruits of high nutritional value and its by-products are sources of bioactive compounds, such as alkaloids. The aim of the study was to investigate how water stress impacts the production of alkaloids. The study was carried out in a nursery, and the knowledge was flood, field capacity and drought. Gas exchange, chlorophyll a fluorescence, antioxidant enzymes, total soluble sugars, starch, reducing sugars, sucrose, total alkaloids and liriodenine were analyzed. We observed that plants subjected to drought had an increase in the production of total alkaloids and liriodenine, without a reduction in photosynthetic metabolism. Plants kept under drought and flood conditions dissipated higher peroxidase activity, while catalase was higher in flooded plants. Starch showed the highest concentration in flooding plants without differing from drought plants; the lowest trehalose concentrations were found in both drought and flooding plants. The drought stimulated the synthesis of total alkaloids and liriodenine without reducing the primary metabolism, which suggests adaptation to Cerrado conditions.
