Preparation and culture of bone marrow-derived macrophages from mice for functional analysis

Gotaro Toda; Toshimasa Yamauchi; Takashi Kadowaki; Kohjiro Ueki

STAR Protocols (Mar 2021)

Preparation and culture of bone marrow-derived macrophages from mice for functional analysis

Gotaro Toda,
Toshimasa Yamauchi,
Takashi Kadowaki,
Kohjiro Ueki

Affiliations

Gotaro Toda: Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan; Corresponding author
Toshimasa Yamauchi: Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan
Takashi Kadowaki: Toranomon Hospital, Tokyo, Japan
Kohjiro Ueki: Department of Molecular Diabetic Medicine, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan; Corresponding author

Journal volume & issue: Vol. 2, no. 1
p. 100246

Abstract

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Summary: The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages.For complete details on the use and execution of this protocol, please refer to Toda et al. (2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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