Electrochemical Immunosensor for NT-proBNP Detection in Artificial Human Saliva: Heart Failure Biomedical Application
Elkahina Ghedir,
Abdoullatif Baraket,
Souad Kouchar,
Selma Rabai,
Messaoud Benounis,
Albert Alcacer,
Angelos Streklas,
Joan Bausells,
Nadia Zine,
Nicole Jaffrezic,
Abdelhamid Errachid
Affiliations
Elkahina Ghedir
Laboratory of Sensors, Instrumentations and Process (LCIP), Institute of Science and Technology, University of Khenchela, Route de El Hamma, Khenchela 40000, Algeria
Abdoullatif Baraket
Institut de Sciences Analytiques (ISA), Université de Lyon—UMR 5280, 5 rue de la Doua, 69100 Lyon, France
Souad Kouchar
Laboratory of Sensors, Instrumentations and Process (LCIP), Institute of Science and Technology, University of Khenchela, Route de El Hamma, Khenchela 40000, Algeria
Selma Rabai
Laboratory of Sensors, Instrumentations and Process (LCIP), Institute of Science and Technology, University of Khenchela, Route de El Hamma, Khenchela 40000, Algeria
Messaoud Benounis
Laboratory of Sensors, Instrumentations and Process (LCIP), Institute of Science and Technology, University of Khenchela, Route de El Hamma, Khenchela 40000, Algeria
Albert Alcacer
Centro Nacional de Microelectronica (IMB-CSIC) Campus UAB, Bellaterra, 08193 Barcelona, Spain
Angelos Streklas
Centro Nacional de Microelectronica (IMB-CSIC) Campus UAB, Bellaterra, 08193 Barcelona, Spain
Joan Bausells
Centro Nacional de Microelectronica (IMB-CSIC) Campus UAB, Bellaterra, 08193 Barcelona, Spain
Nadia Zine
Institut de Sciences Analytiques (ISA), Université de Lyon—UMR 5280, 5 rue de la Doua, 69100 Lyon, France
Nicole Jaffrezic
Institut de Sciences Analytiques (ISA), Université de Lyon—UMR 5280, 5 rue de la Doua, 69100 Lyon, France
Abdelhamid Errachid
Institut de Sciences Analytiques (ISA), Université de Lyon—UMR 5280, 5 rue de la Doua, 69100 Lyon, France
Monitoring of circulating N-terminal proBNP (NT-proBNP) biomarkers is crucial for the diagnosis of people suffering from heart failure (HF). In this work, we describe a novel ultra-sensitive NT-proBNP immunosensor for NT-proBNP detection in artificial human saliva. The surface of the developed immunosensor based on gold working microelectrodes (WEs) was biofunctionalized through carboxyl diazonium to immobilize anti-NT-proBNP antibodies. The chemical surface modification of WEs was carried out by cyclic voltammetery CV whilst the quantification of NT-proBNP biomarkers was made by electrochemical impedance spectroscopy (EIS). The immunosensor has demonstrated a linear detection response within the range 1–20 pg/mL for NTproBNP detection in artificial human saliva with a good selectivity in the presence of other interferences.
