Effect of severe environmental thermal stress on redox state in salmon

Abstract

Fish are exposed to many kinds of environmental stressors and the chances of succumbing to infectious diseases may be increased a result. For example, an acute increase in temperature can induce numerous physiological changes in the body. In the present study, we examined the redox state in response to a severe acute stress resulting from heat shock in teleost coho salmon (Oncorhynchus kisutch). The plasma lipid peroxides levels in fish gradually increased after heat shock treatment. By 2.5 h post-heat stress, plasma glutathione (GSH) levels had decreased, but they had returned to basal levels by 17.5 h post-stress. Plasma superoxide dismutase activities in stressed fish were significantly increased compared with those in control fish at 17.5 h post-stress, but had returned to basal levels by 48 h post-stress. Expression levels of hepatic GSH and heat shock protein 70 gradually increased after heat shock treatment. These results concerning the changing patterns of multiple important redox-related biomarkers suggest that severe thermal stressors can affect the redox state and induce oxidative stress in ectothermal animals, such as fish, in vivo. Hence, manipulation of appropriate thermal treatment may possibly be useful to control fish fitness.

Keywords

• Redox state
• Thermal stressor
• Heat shock
• Oxidative stress
• Fish
• Coho salmon