TOXICOLOGICAL AND HYGIENIC ASSESSMENT OF THE NEW ACTIVE INGREDIENT OF THE AVERSECTINS CLASS - MILBEMECTIN AND THE FORMULATION BASED ON IT MILBEKNOK 1%, CE

Abstract

Relevance. The priorities of modern agricultural production are the application of new pesticides that are highly effective, safe for humans and do not have a negative impact on environmental objects. The most promising is the synthesis of substances from natural materials or their synthetic analogues (pesticides of biological origin). These include avermectin class substance – milbemectin. Objective of the work was the toxicological and hygienic evaluation of the new active ingredient of the aversectins class - milbemectin and the formulation based on it Milbeknok 1%, CE, substantiation of the acceptable daily dose (ADD) of milbemectin. Materials and methods. Expert-analytical, toxicological and hygienic methods, analysis of literature data were used during the research. The hygienic assessment of the toxicity of milbemectin and the formulation based on it was carried out in accordance with State Standard 8.8.1.002-98 “Hygienic classification of pesticides by degree of hazard”. Results. The analysis of the acute toxicity of the formulation and its active ingredient showed that Milbeknok 1%, CE, according to State Standard 8.8.1.002-98, belongs to the 3rd class of hazard, and milbemectin – to the 2nd class, according to the limiting criterion – acute inhalation toxicity. In short-term and long-term experiments with rats, mice and dogs, the main effect of milbemectin exposure was general toxicity and hepatotoxicity. According to carcinogenicity, reproductive and embryotoxicity, milbemectin is pertained to the 3rd class of hazard. Substantiating the ADD of milbemectin we used the smallest NO(A)EL value of 3 mg/kg, established for the general toxic effect in the experiment to study the chronic effect on dogs and safety factor of 1000 (taking into account the lack of a study of developmental neurotoxicity and limited data on reproductive toxicity of the compound): ADD = 3/1000 = 0.003 mg/kg. Conclusions. It was established that the Milbeknok 1%, CE pertains to the 3rd class, and its active substance - to the 2nd class of hazard, according to the limiting criterion – acute inhalation toxicity. The substantiated ADD value of milbemectin is 0.003 mg/kg.

Keywords

• milbemectin, toxicity, acceptable daily dose