Nature Communications (Nov 2017)
Novel genetically encoded fluorescent probes enable real-time detection of potassium in vitro and in vivo
- Helmut Bischof,
- Markus Rehberg,
- Sarah Stryeck,
- Katharina Artinger,
- Emrah Eroglu,
- Markus Waldeck-Weiermair,
- Benjamin Gottschalk,
- Rene Rost,
- Andras T. Deak,
- Tobias Niedrist,
- Nemanja Vujic,
- Hanna Lindermuth,
- Ruth Prassl,
- Brigitte Pelzmann,
- Klaus Groschner,
- Dagmar Kratky,
- Kathrin Eller,
- Alexander R. Rosenkranz,
- Tobias Madl,
- Nikolaus Plesnila,
- Wolfgang F. Graier,
- Roland Malli
Affiliations
- Helmut Bischof
- Institute of Molecular Biology and Biochemistry, Medical University of Graz
- Markus Rehberg
- Ludwig-Maximilians University (LMU), Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München
- Sarah Stryeck
- Institute of Molecular Biology and Biochemistry, Medical University of Graz
- Katharina Artinger
- Clinical Division of Nephrology, Medical University of Graz
- Emrah Eroglu
- Institute of Molecular Biology and Biochemistry, Medical University of Graz
- Markus Waldeck-Weiermair
- Institute of Molecular Biology and Biochemistry, Medical University of Graz
- Benjamin Gottschalk
- Institute of Molecular Biology and Biochemistry, Medical University of Graz
- Rene Rost
- Institute of Molecular Biology and Biochemistry, Medical University of Graz
- Andras T. Deak
- Clinical Division of Nephrology, Medical University of Graz
- Tobias Niedrist
- Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz
- Nemanja Vujic
- Institute of Molecular Biology and Biochemistry, Medical University of Graz
- Hanna Lindermuth
- Institute of Biophysics, Medical University of Graz
- Ruth Prassl
- Institute of Biophysics, Medical University of Graz
- Brigitte Pelzmann
- Institute of Biophysics, Medical University of Graz
- Klaus Groschner
- Institute of Biophysics, Medical University of Graz
- Dagmar Kratky
- Institute of Molecular Biology and Biochemistry, Medical University of Graz
- Kathrin Eller
- Clinical Division of Nephrology, Medical University of Graz
- Alexander R. Rosenkranz
- Clinical Division of Nephrology, Medical University of Graz
- Tobias Madl
- Institute of Molecular Biology and Biochemistry, Medical University of Graz
- Nikolaus Plesnila
- Ludwig-Maximilians University (LMU), Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München
- Wolfgang F. Graier
- Institute of Molecular Biology and Biochemistry, Medical University of Graz
- Roland Malli
- Institute of Molecular Biology and Biochemistry, Medical University of Graz
- DOI
- https://doi.org/10.1038/s41467-017-01615-z
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 12
Abstract
K+ plays an important role in physiology and disease, but the lack of high specificity K+ sensors limits our understanding of its spatiotemporal dynamics. Here the authors develop genetically-encoded FRET-based probes able to quantify K+ concentration in body fluids, cells and specific organelles.
