An imaging flow cytometry-based methodology for the analysis of single extracellular vesicles in unprocessed human plasma

Affiliations

Wouter W. Woud: Erasmus MC Transplant Institute, Department of Internal Medicine, University Medical Center Rotterdam
Edwin van der Pol: Biomedical Engineering & Physics, Laboratory Experimental Clinical Chemistry, Vesicle Observation Center, Amsterdam UMC, University of Amsterdam
Erik Mul: Department Central Cell Analysis Facility, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam
Martin J. Hoogduijn: Erasmus MC Transplant Institute, Department of Internal Medicine, University Medical Center Rotterdam
Carla C. Baan: Erasmus MC Transplant Institute, Department of Internal Medicine, University Medical Center Rotterdam
Karin Boer: Erasmus MC Transplant Institute, Department of Internal Medicine, University Medical Center Rotterdam
Ana Merino: Erasmus MC Transplant Institute, Department of Internal Medicine, University Medical Center Rotterdam

Abstract

A method to quantitate and phenotype Extracellular Vesicles (EVs) in unprocessed human plasma using Imaging Flow Cytometry is presented.