High-Frequency, High-Throughput Quantification of SARS-CoV-2 RNA in Wastewater Settled Solids at Eight Publicly Owned Treatment Works in Northern California Shows Strong Association with COVID-19 Incidence

Marlene K. Wolfe; Aaron Topol; Alisha Knudson; Adrian Simpson; Bradley White; Duc J. Vugia; Alexander T. Yu; Linlin Li; Michael Balliet; Pamela Stoddard; George S. Han; Krista R. Wigginton; Alexandria B. Boehm

doi:10.1128/mSystems.00829-21

mSystems (Oct 2021)

High-Frequency, High-Throughput Quantification of SARS-CoV-2 RNA in Wastewater Settled Solids at Eight Publicly Owned Treatment Works in Northern California Shows Strong Association with COVID-19 Incidence

Marlene K. Wolfe,
Aaron Topol,
Alisha Knudson,
Adrian Simpson,
Bradley White,
Duc J. Vugia,
Alexander T. Yu,
Linlin Li,
Michael Balliet,
Pamela Stoddard,
George S. Han,
Krista R. Wigginton,
Alexandria B. Boehm

Affiliations

Marlene K. Wolfe: Department of Civil & Environmental Engineering, Stanford University, Stanford, California, USA
Aaron Topol: Verily Life Sciences, South San Francisco, California, USA
Alisha Knudson: Verily Life Sciences, South San Francisco, California, USA
Adrian Simpson: Verily Life Sciences, South San Francisco, California, USA
Bradley White: Verily Life Sciences, South San Francisco, California, USA
Duc J. Vugia: California Department of Public Health, Infectious Diseases Branch, Richmond, California, USA
Alexander T. Yu: California Department of Public Health, Infectious Diseases Branch, Richmond, California, USA
Linlin Li: County of Santa Clara Public Health Department, San Jose, California, USA
Michael Balliet: County of Santa Clara Department of Environmental Health, San Jose, California, USA
Pamela Stoddard: County of Santa Clara Public Health Department, San Jose, California, USA
George S. Han: County of Santa Clara Public Health Department, San Jose, California, USA
Krista R. Wigginton: ORCiD; Department of Civil & Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA
Alexandria B. Boehm: ORCiD; Department of Civil & Environmental Engineering, Stanford University, Stanford, California, USA

DOI: https://doi.org/10.1128/mSystems.00829-21
Journal volume & issue: Vol. 6, no. 5

Abstract

Read online

Access to reliable, rapid monitoring data is critical to guide response to an infectious disease outbreak. For pathogens that are shed in feces or urine, monitoring wastewater can provide a cost-effective snapshot of transmission in an entire community via a single sample.

Published in mSystems

ISSN: 2379-5077 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/msystems

About the journal