Nature Communications (Sep 2019)
Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle
- Philip J. M. Brouwer,
- Aleksandar Antanasijevic,
- Zachary Berndsen,
- Anila Yasmeen,
- Brooke Fiala,
- Tom P. L. Bijl,
- Ilja Bontjer,
- Jacob B. Bale,
- William Sheffler,
- Joel D. Allen,
- Anna Schorcht,
- Judith A. Burger,
- Miguel Camacho,
- Daniel Ellis,
- Christopher A. Cottrell,
- Anna-Janina Behrens,
- Marco Catalano,
- Iván del Moral-Sánchez,
- Thomas J. Ketas,
- Celia LaBranche,
- Marit J. van Gils,
- Kwinten Sliepen,
- Lance J. Stewart,
- Max Crispin,
- David C. Montefiori,
- David Baker,
- John P. Moore,
- Per Johan Klasse,
- Andrew B. Ward,
- Neil P. King,
- Rogier W. Sanders
Affiliations
- Philip J. M. Brouwer
- Amsterdam UMC, Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, University of Amsterdam
- Aleksandar Antanasijevic
- Department of Integrative Structural and Computational Biology, The Scripps Research Institute
- Zachary Berndsen
- Department of Integrative Structural and Computational Biology, The Scripps Research Institute
- Anila Yasmeen
- Department of Microbiology and Immunology, Weill Medical College of Cornell University
- Brooke Fiala
- Department of Biochemistry, University of Washington
- Tom P. L. Bijl
- Amsterdam UMC, Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, University of Amsterdam
- Ilja Bontjer
- Amsterdam UMC, Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, University of Amsterdam
- Jacob B. Bale
- Department of Biochemistry, University of Washington
- William Sheffler
- Department of Biochemistry, University of Washington
- Joel D. Allen
- Biological Sciences and Institute of Life Sciences, University of Southampton
- Anna Schorcht
- Amsterdam UMC, Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, University of Amsterdam
- Judith A. Burger
- Amsterdam UMC, Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, University of Amsterdam
- Miguel Camacho
- Amsterdam UMC, Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, University of Amsterdam
- Daniel Ellis
- Department of Biochemistry, University of Washington
- Christopher A. Cottrell
- Department of Integrative Structural and Computational Biology, The Scripps Research Institute
- Anna-Janina Behrens
- Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford
- Marco Catalano
- Amsterdam UMC, Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, University of Amsterdam
- Iván del Moral-Sánchez
- Amsterdam UMC, Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, University of Amsterdam
- Thomas J. Ketas
- Department of Microbiology and Immunology, Weill Medical College of Cornell University
- Celia LaBranche
- Department of Surgery, Duke University Medical Center
- Marit J. van Gils
- Amsterdam UMC, Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, University of Amsterdam
- Kwinten Sliepen
- Amsterdam UMC, Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, University of Amsterdam
- Lance J. Stewart
- Department of Biochemistry, University of Washington
- Max Crispin
- Biological Sciences and Institute of Life Sciences, University of Southampton
- David C. Montefiori
- Department of Surgery, Duke University Medical Center
- David Baker
- Department of Biochemistry, University of Washington
- John P. Moore
- Department of Microbiology and Immunology, Weill Medical College of Cornell University
- Per Johan Klasse
- Department of Microbiology and Immunology, Weill Medical College of Cornell University
- Andrew B. Ward
- Department of Integrative Structural and Computational Biology, The Scripps Research Institute
- Neil P. King
- Department of Biochemistry, University of Washington
- Rogier W. Sanders
- Amsterdam UMC, Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, University of Amsterdam
- DOI
- https://doi.org/10.1038/s41467-019-12080-1
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 17
Abstract
Nanoparticles are a promising approach to increase immunogenicity of protein antigens for vaccines. Here, Brouwer et al. design self-assembling, two-component protein NPs that present native-like SOSIP trimers of HIV envelope protein and determine immunogenicity in a small animal model.
