Genetic and biological characterisation of a Grapevine virus A isolate from the Czech Republic

Abstract

An isolate of Grapevine virus A (GVA) from the Czech Republic was obtained from the grapevine cultivar Müller Thurgau. Symptoms of GVA - Kober stem grooving disease were not observed in the infected grapevines (which had been grafted onto Kober 5BB rootstock). A partial genomic sequence of the GVA isolate, 1523 nucleotides long, was obtained. The sequence completely covers the genes for both a movement and a coat protein. Compared to the GVA sequences available in databases, the nucleotide identity reached 84%. The amino acid identity in the movement protein reached 88%, and 98% in the coat protein.

Keywords

• grapevine virus a
• grapevine
• movement protein
• coat protein
• phylogenetic analysis
• sequencing