Frontiers in Cellular and Infection Microbiology (Oct 2024)
Identification of porcine PARP11 as a restricted factor for pseudorabies virus
- Chunyun Qi,
- Dehua Zhao,
- Xi Wang,
- Lanxin Hu,
- Yao Wang,
- Heyong Wu,
- Feng Li,
- Jian Zhou,
- Tianyi Zhang,
- Aosi Qi,
- Yuran Huo,
- Qiuse Tu,
- Shuyu Zhong,
- Hongming Yuan,
- Hongming Yuan,
- Hongming Yuan,
- Dongmei Lv,
- Dongmei Lv,
- Dongmei Lv,
- Shouqing Yan,
- Hongsheng Ouyang,
- Hongsheng Ouyang,
- Hongsheng Ouyang,
- Hongsheng Ouyang,
- Daxin Pang,
- Daxin Pang,
- Daxin Pang,
- Zicong Xie,
- Zicong Xie,
- Zicong Xie
Affiliations
- Chunyun Qi
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Dehua Zhao
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Xi Wang
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Lanxin Hu
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Yao Wang
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Heyong Wu
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Feng Li
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Jian Zhou
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Tianyi Zhang
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Aosi Qi
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Yuran Huo
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Qiuse Tu
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Shuyu Zhong
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Hongming Yuan
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Hongming Yuan
- Chongqing Research Institute, Jilin University, Chongqing, China
- Hongming Yuan
- Center for Animal Science and Technology Research, Chongqing Jitang Biotechnology Research Institute Co., Ltd, Chongqing, China
- Dongmei Lv
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Dongmei Lv
- Chongqing Research Institute, Jilin University, Chongqing, China
- Dongmei Lv
- Center for Animal Science and Technology Research, Chongqing Jitang Biotechnology Research Institute Co., Ltd, Chongqing, China
- Shouqing Yan
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Hongsheng Ouyang
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Hongsheng Ouyang
- Chongqing Research Institute, Jilin University, Chongqing, China
- Hongsheng Ouyang
- Center for Animal Science and Technology Research, Chongqing Jitang Biotechnology Research Institute Co., Ltd, Chongqing, China
- Hongsheng Ouyang
- Laboratory of Biotechnology and Biomedical Research, Shenzhen Kingsino Technology Co., Ltd., Shenzhen, China
- Daxin Pang
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Daxin Pang
- Chongqing Research Institute, Jilin University, Chongqing, China
- Daxin Pang
- Center for Animal Science and Technology Research, Chongqing Jitang Biotechnology Research Institute Co., Ltd, Chongqing, China
- Zicong Xie
- Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, Jilin, China
- Zicong Xie
- Chongqing Research Institute, Jilin University, Chongqing, China
- Zicong Xie
- Center for Animal Science and Technology Research, Chongqing Jitang Biotechnology Research Institute Co., Ltd, Chongqing, China
- DOI
- https://doi.org/10.3389/fcimb.2024.1414827
- Journal volume & issue
-
Vol. 14
Abstract
IntroductionPRV infection in swine can cause devastating disease and pose a potential threat to humans. Advancing the interplay between PRV and host is essential to elucidate the pathogenic mechanism of PRV and identify novel anti-PRV targets.MethodsPARP11-KO PK-15 cells were firstly constructed by CRISPR/Cas9 technology. Next, the effect of PARP11-KO on PRV infection was determined by RT-qPCR, TCID50 assay, RNA-seq, and western blot.Results and discussionIn this study, we identified PARP11 as a host factor that can significantly affect PRV infection. Inhibition of PARP11 and knockout of PARP11 can significantly promoted PRV infection. Subsequently, we further found that PARP11 knockout upregulated the transcription of NXF1 and CRM1, resulting in enhanced transcription of viral genes. Furthermore, we also found that PARP11 knockout could activate the autophagy pathway and suppress the mTOR pathway during PRV infection. These findings could provide insight into the mechanism in which PARP11 participated during PRV infection and offer a potential target to develop anti-PRV therapies.
Keywords
