Genetic diversity of Escherichia coli harboring virulence gene Stx1 and Stx2 isolated from common carp fish in Nineveh Governorate using ERIC-PCR

Abstract

The goal of this study is to discover the genetic diversity of twelve Escherichia coli isolates harbor virulence genes (Stx1 and Stx2) isolated from farm fish and local market fish in different locations of Nineveh Governorate using Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) technique in the time span November, 2021 - January, 2022. The source of contamination was found depending on the fingerprint analysis of the repetitive polymerase chain reaction among the genes of the intestinal micro-organism ERIC-PCR for every isolate. The results show that there are similarities among the isolates and it ranged about 51%-100%. The isolates are divided into 7 genotypes, based primarily on a 90% similarity threshold 1 to 7, with the most offspring being inside genotype 1, since genotype 1 used to be the biggest team containing four isolates. Then, two isolates are grouped under genotypes 3 and 5. In contrast, genotype 2, 4, 6 and 7 have only one isolate. By virtue of genetic variations in the location, there was once an excessive clonal dispersion of most E. coli strains. In addition, the study shows genetic differences in E. coli in 83.3%, (10/12) of the isolates, in the different geographical locations of Mosul city. The E. coli isolates were classified into some groups: first, different genetic grouping, different geographical locations, same genotype; second, different genetic grouping, same geographical locations, different genotypes.

Keywords

• eric-pcr
• fish farms
• local fish markets
• virulence gene
• e. coli