Construction of Fingerprint Map of 12 Varieties Based on SSR Markers and Identification of Hybrid F1 Progenies in Gerbera hybrida

Abstract

【Objective】Based on the method of SSR molecular markers, this study conducted phylogenetic analysis on 12 germplasms and identified true and false hybrids from F1 hybrid progenies of some germplasms in Gerbera hybrida, in order to offer a technological support for the evaluation and innovative utilization of germplasm resources in G. hybrida.【Method】Twelve Gerbera hybrida germplasm resources were collected and their genomic DNA was extracted. PCR amplification was performed using 50 pairs of SSR primers. Cluster analysis and fingerprint construction were conducted by detecting the results of related polymorphisms, as well as identification of hybrid F1 offspring of some varieties.【Result】A total of 74 polymorphic alleles were amplified by 18 pairs of primers in 12 varieties of G. hybrida, with an average of 4.1 polymorphic alleles amplified by each pair of primers. A phylogenetic tree of 12 germplasm resources was constructed using 74 alleles in G. hybrida. UPGMA clustering analysis results showed that when the genetic coefficient was 0.75, the tested 12 germplasm resources could be divided into 5 groups, with Group Ⅰ including 'Yunnanhong', 'Da088', and 'Qingren'. Group Ⅱ including 'Fenjiaren', 'Fenqiu', 'Zhenai', and 'Fuwa'. Group Ⅲ including 'Xiuse', 'Shunv', and 'Huangqiu', both Group Ⅳ and Group Ⅴ containing only one variety, namely 'Zishuijing' and 'Shenzhen 5', respectively. Four pairs of primers were screened, including GHSSR-1, GHSSR-18, GHSSR-20, and GHSSR-21, which can completely distinguish the 12 germplasms with each other. Among them, the last three pairs of primers identified lines with parental specific heterotopies as Gh-5, Gh-4, and Gh-1, respectively. The identification rate of a single pair of primers was 33%. All three tested individual plants are true hybrids, with a true hybrid rate of 100%.【Conclusion】Twelve fingerprint maps of G.hybrida varieties were successfully constructed. At the same time, 3 hybrid F1 offspring seedlings were identified as true hybrids, providing dependable SSR marker primers for the evaluation and innovative utilization of resources in G. hybrida.

Keywords

• gerbera hybrida
• ssr
• capillary electrophoresis
• relationship analysis
• fingerprint map
• identification of hybrida