Construction, expression, and characterization of AG11–843 and AG11–1581
Xie Yan,
Yan-Tao Yang,
Wei Shi,
Xia Ai,
Xu-Guang Xi
Affiliations
Xie Yan
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China
Yan-Tao Yang
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China
Wei Shi
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China
Xia Ai
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China
Xu-Guang Xi
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China; Laboratoire de Biologie et Pharmacologie Appliquée, Ecole Normals Supérieure de Cachan, CNRS, 61 Avenue du Président Wilson, 94235 Cachan, France; Corresponding author at: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
This data article contains descriptive and experimental data on the construction, expression, and simple characterization of AG11–843 and AG11–1581. AG1 is an important member of the DUF1220 protein family. It׳s hard to get the recombinant protein because of its DNA sequence. The DNA sequence were optimized by proper design, cloned by overlap PCR and constructed into expression vector. AG11–843 and AG11–1581.were over expressed in Escherichia coli, purified and analyzed by dynamic light scattering and gel filtration analysis. An effective technique is provided to construct and express proteins with complicated sequences. Keywords: Gene synthesis, Plasmid construction, PCR, Recombinant protein expression, DUF1220, AG1
