Immune effects of partially purified polyamine oxidase from cerebrospinal fluid against infection with secondary hydatid disease in mice.III.Growth and development of hydatid cysts .

Abstract

The study included immune response to infection with secondary hydatid disease in BALB/c mice activated by partially purified Cerebrospinal fluid-polyamine oxidase CSF-P AO with spermine (Spm) and infected with protoscoleces of Echinococcus granulosus. The pathological changes occurred in mice activated by differentconcentrations of PA0(200-1600Jlg/10gm body weight) with constant concentration (200 jlgl 1 Ogm body weight) of substrate (spermine), and by the same concentration of spermine alone, were followed in comparison with positive control group along one month, depending on many criteria.These criteria included changes in numbers, weights and diameters of hydatid cysts, and their percentage eduction. In addition, changes in weights of liver and spleen and their organ indices .The results revealedan obvious decrease in numbers, weights and diameters of secondary hydatid cysts grown for one month in activated mice, in comparison with the +ve control group, supported by elevation in the percentage of reduction of hydatid cysts in activated mice.A decrease in the mean of liver and spleen weights, and a variation in organ indices in activated mice occurred within one month, whereas no change was observed in the mean of spleen weight, in comparison with +ve control group.Therefore, it may be concluded here that P AO isolated from CSF with Spm could be considered as an effective immunumodulator against infection with secondary hydatid disease.

Keywords

• polyamine oxidase
• cerebrospinal fluid
• hydatid disease
• mice