Frontiers in Microbiology (Mar 2016)

The IncP-6 plasmid p10265-KPC from Pseudomonas aeruginosa carries a novel ΔISEc33-associated blaKPC-2 gene cluster

  • Xiaotian eDai,
  • Dongsheng eZhou,
  • Wei eXiong,
  • Jiao eFeng,
  • Wenbo eLuo,
  • Guangming eLuo,
  • Haijing eWang,
  • Fengjun eSun,
  • Xiangdong eZhou

DOI
https://doi.org/10.3389/fmicb.2016.00310
Journal volume & issue
Vol. 7

Abstract

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Pseudomonas aeruginosa strain 10265 was recovered from a patient with pneumonia in a Chinese public hospital, and it displays the carbapenem resistance phenotype due to the acquisition of a nonconjugative but mobilizable IncP-6-type plasmid p10265-KPC. p10265-KPC carries a Tn5563-borne defective mer locus and a novel ΔISEc33-associated blaKPC-2 gene cluster without paired invert repeats and direct repeats at both ends. Mobilization of this ΔISEc33-associated element in p10265-KPC would attribute to homologous recombination-based insertion of a foreign structure Tn3-ISApu1-orf7-ISApu2- ISKpn27-ΔblaTEM-1-blaKPC-2-ΔISKpn6-korC-orf6-klcA-ΔrepB into a pre-existent intact ISEc33, making ISEc33 truncated at the 3' end. The previously reported pCOL-1 represents the first sequenced KPC-producing IncP-6 plasmid, while p10265-KPC is the second one. These two plasmids carry two distinct blaKPC-2 gene clusters, which are inserted into the different sites of the IncP-6 backbone and have different evolutionary histories of assembly and mobilization. This is also the first report of identification of the IncP-6-type resistance plasmid in China.

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