Ukrainian Scientific Medical Youth Journal (Apr 2016)
VALUE INNATE IMMUNITY (TOLL-LIKE RECEPTORS 2, 4 , 9 ) IN THE PATHOGENESIS OF PSORIASIS
Abstract
Purpose of the work - to investigate the mechanisms of local immune inflammation in psoriasis, taking into account changes immunohistochemikal expression of Toll-like receptor 2, 4, 9 (TLR2, 4, 9) in patients with this dermatosis before and after systemic immunosuppressive therapy. Materials and Methods. Immunohistochemical research of biopsy material, taken from areas of skin psoriasis pouring out at 62 patients on psoriasis. In addition, for comparison of results of immunogistochemikal research a study of biopsy material of skin of front abdominal wall is undertaken for the persons of corresponding age without psoriasis (5 patients) that was execute the operation of hernioplastick. For find character and prevalence of local cellular immune and inflammatory reactions immunohistochemical methodologies were used for the exposure of expression of marker of TLR2, 4, 9. Results. While setting the immunohistochemical reaction in order to define the character for TLR2, TLR4 and TLR9 expression in the skin in the norm, we found out the diffuse cytoplasmic and nuclear coloration of epidermis cells, vessels endothelium and singular macrophage cells. The positive reaction with nuclear patterns of expression in epithelial cells and dendrite cells of epidermis basal layer was observed at patients with psoriasis in the areas of intact skin before treatment while setting the TLR2 immunohistochemical reaction. More intensive and widespread nuclear and cytoplasmic coloration of epidermis cells is observed while setting the reaction of TLR4 expression. The background diffuse cytoplasmic and nuclear coloration of epidermis cells, vessels endothelium and singular macrophage cells is also observed at study of TLR4 expression in the areas of skin psoriaform. Less intensive diffuse background coloration of epidermis, vessels endothelium and some cells in inflammatory skin infiltrates is found while setting TLR2 and TLR9 immunohistochemical reaction. The intensity of TLR2 and TLR4 immunohistochemical reaction is a bit reduced after the treatment, performed in intact skin. The positive background nuclear coloration of epithelial cells in the suface layers of epidermis is observed in the skin of patients with psoriasis after performed treatment unlike the skin in the norm. Positive painted macrophages are perivasculary found in a small quantity in depper layers of the skin. Conclusions. The changes revealed immunohistochemical expression of TLR 2, TLR4 and TLR9 plots in psoriatic skin rash in patients with psoriasis in the dynamics of treatment indicate a certain value of the corresponding receptor in the development of immune inflammation in the skin in psoriasis, and may also serve as an additional diagnostic and prognostic marker for the clinical course of the nature of the dermatosis. In addition, expression of TLR2 identified changes in the psoriatic skin rash areas expand understanding of the pathogenesis of psoriasis.
