Nature Communications (Apr 2020)

A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues

  • Jaime Andrés Rivas-Pardo,
  • Yong Li,
  • Zsolt Mártonfalvi,
  • Rafael Tapia-Rojo,
  • Andreas Unger,
  • Ángel Fernández-Trasancos,
  • Elías Herrero-Galán,
  • Diana Velázquez-Carreras,
  • Julio M. Fernández,
  • Wolfgang A. Linke,
  • Jorge Alegre-Cebollada

DOI
https://doi.org/10.1038/s41467-020-15465-9
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 13

Abstract

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Testing mechanical forces on native molecules in natural environments remains a challenge. Here the authors engineer titin to carry a HaloTag-TEV insertion to allow analysis of dynamics under force in muscle fibers.