Frontiers in Immunology (Dec 2022)
Placental galectins regulate innate and adaptive immune responses in pregnancy
- Orsolya Oravecz,
- Orsolya Oravecz,
- Roberto Romero,
- Roberto Romero,
- Roberto Romero,
- Roberto Romero,
- Roberto Romero,
- Eszter Tóth,
- Judit Kapitány,
- Máté Posta,
- Máté Posta,
- Dahiana M. Gallo,
- Dahiana M. Gallo,
- Dahiana M. Gallo,
- Simona W. Rossi,
- Adi L. Tarca,
- Adi L. Tarca,
- Adi L. Tarca,
- Adi L. Tarca,
- Offer Erez,
- Offer Erez,
- Offer Erez,
- Offer Erez,
- Zoltán Papp,
- Zoltán Papp,
- János Matkó,
- Nándor Gábor Than,
- Nándor Gábor Than,
- Nándor Gábor Than,
- Nándor Gábor Than,
- Nándor Gábor Than,
- Andrea Balogh
Affiliations
- Orsolya Oravecz
- Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary
- Orsolya Oravecz
- Doctoral School of Biology, Institute of Biology, ELTE Eötvös Loránd University, Budapest, Hungary
- Roberto Romero
- Perinatology Research Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, United States Department of Health and Human Services, Detroit, MI, United States
- Roberto Romero
- Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, United States
- Roberto Romero
- Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, MI, United States
- Roberto Romero
- Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, United States
- Roberto Romero
- Detroit Medical Center, Detroit, MI, United States
- Eszter Tóth
- Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary
- Judit Kapitány
- Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary
- Máté Posta
- Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary
- Máté Posta
- Károly Rácz Doctoral School of Clinical Medicine, Semmelweis University, Budapest, Hungary
- Dahiana M. Gallo
- Perinatology Research Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, United States Department of Health and Human Services, Detroit, MI, United States
- Dahiana M. Gallo
- Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, United States
- Dahiana M. Gallo
- 0Department of Obstetrics and Gynecology, Universidad Del Valle, Cali, Colombia
- Simona W. Rossi
- 1Genesis Theranostix Group, Budapest, Hungary
- Adi L. Tarca
- Perinatology Research Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, United States Department of Health and Human Services, Detroit, MI, United States
- Adi L. Tarca
- Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, United States
- Adi L. Tarca
- Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, United States
- Adi L. Tarca
- 1Genesis Theranostix Group, Budapest, Hungary
- Offer Erez
- Perinatology Research Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, United States Department of Health and Human Services, Detroit, MI, United States
- Offer Erez
- Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, United States
- Offer Erez
- 1Genesis Theranostix Group, Budapest, Hungary
- Offer Erez
- 2Department of Obstetrics and Gynecology, Soroka University Medical Center, Beer Sheva, Israel
- Zoltán Papp
- 3Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary
- Zoltán Papp
- 4Maternity Private Clinic of Obstetrics and Gynecology, Budapest, Hungary
- János Matkó
- Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary
- Nándor Gábor Than
- Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary
- Nándor Gábor Than
- Perinatology Research Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, United States Department of Health and Human Services, Detroit, MI, United States
- Nándor Gábor Than
- 1Genesis Theranostix Group, Budapest, Hungary
- Nándor Gábor Than
- 3Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary
- Nándor Gábor Than
- 4Maternity Private Clinic of Obstetrics and Gynecology, Budapest, Hungary
- Andrea Balogh
- Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary
- DOI
- https://doi.org/10.3389/fimmu.2022.1088024
- Journal volume & issue
-
Vol. 13
Abstract
IntroductionGalectins are master regulators of maternal immune responses and placentation in pregnancy. Galectin-13 (gal-13) and galectin-14 (gal-14) are expressed solely by the placenta and contribute to maternal-fetal immune tolerance by inducing the apoptosis of activated T lymphocytes and the polarization of neutrophils toward an immune-regulatory phenotype.Furthermore, their decreased placental expression is associated with pregnancy complications, such as preeclampsia and miscarriage. Yet, our knowledge of the immunoregulatory role of placental galectins is incomplete.MethodsThis study aimed to investigate the effects of recombinant gal-13 and gal-14 on cell viability, apoptosis, and cytokine production of peripheral blood mononuclear cells (PBMCs) and the signaling pathways involved.ResultsHerein, we show that gal-13 and gal-14 bind to the surface of non-activated PBMCs (monocytes, natural killer cells, B cells, and T cells) and increase their viability while decreasing the rate of their apoptosis without promoting cell proliferation. We also demonstrate that gal-13 and gal-14 induce the production of interleukin (IL)-8, IL-10, and interferon-gamma cytokines in a concentration-dependent manner in PBMCs. The parallel activation of Erk1/2, p38, and NF-ĸB signaling evidenced by kinase phosphorylation in PBMCs suggests the involvement of these pathways in the regulation of the galectin-affected immune cell functions.DiscussionThese findings provide further evidence on how placenta-specific galectins assist in the establishment and maintenance of a proper immune environment during a healthy pregnancy.
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