Improving High Light Tolerance of Tobacco Plants: Adequate Magnesium Supply Enhances Photosynthetic Performance

Abstract

High light (HL) significantly impacts plant photosynthesis. This study investigated the effects of different magnesium (Mg) levels (0, 1, 2, and 5 mol Mg plant−1; HMg0, HMg1, HMg2, and HMg5) on tobacco (Nicotiana tabacum L. cv. Cuibi No. 1) under HL (1500 μmol m−2 s−1), aiming to understand the role of Mg in mitigating the impact of HL on photosynthesis and carbon–nitrogen metabolism. Plants treated with 1 mol Mg plant−1 under 750 μmol m−2 s−1 light conditions served as the control. HL led to a reduced chlorophyll (Chl) content and inhibited the maximum photosynthetic rate (Pmax). It also decreased energy involved in photosynthetic electron transfer (ET) and electron flux to reduction end-electron acceptors at the photosystems I (PSI) acceptor side (RE) and caused photosynthetic system damage. H2O2 accumulation exacerbated membrane lipid peroxidation damage, disrupting carbon and nitrogen metabolism, and inducing antioxidant enzyme activity. HMg2 increased Chl content, stomatal conductance, intercellular CO2 concentration, and the net photosynthetic rate compared to HMg0. It enhanced ET efficiency, PSI and PSII functionality, reduced dissipated energy flux (DI), and minimized photosynthesis damage. Conversely, excessive Mg application (HMg5) decreased Pmax and PSII activity, increasing DI. Adequate Mg supply alleviated HL’s detrimental effects by enhancing Chl content and ET and RE efficiency.

Keywords

• high light intensity
• magnesium deficiency
• photosynthetic fluorescence characteristic
• carbon and nitrogen metabolism