The Preventive Effect of Thymoquinone and Ceratonia Siliqua L. in Experimental Asthmatic Pregnant Rats: Histologically and Immunohistochemically Evaluation

Abstract

T here are no studies yet have been compared pregnant rat asthma models by assessing the airway both histologically and immunohistochemically under the effect of Thymoquinone TQ and Ceratonia siliqua CS Carob . To compare asthmatic pregnant models with TQ and CS by studying changes of both the histologically and the immunohistochemically of lung tissue using light microscopy. 18 female wistar rats were randomly split to 3 groups: asthmatic pregnant group I sensitized by intraperitoneal ovalbumin injection OVA with alum on days 0 and 14 and submitted to OVA aerosolized 3 days in excess of the next 1 week then they coupled with male rats to get pregnancy; asthmatic pregnant with TQ and CS group II sensitized as above then they administered each of TQ and CS on the last 5 days of pregnancy; and asthmatic pregnant with dexamethasone group III that received dexamethasone by intraperitoneal injection on last 5 days of pregnancy.Lung tissue immunohistochemically and histologically were evaluated. Various histological changes in lung tissues of group I were revealed. However, treatment of TQ and CSwas prevented these changes in group II. Immunohistochemically, vascular endothelialgrowth factor VEGF staining levels increase was observed in group I, while there was asignificant decrease in group II than it is in group III. In this study, the use of TQ and CS has been shown to alleviate the histological changes and immunohistochemically causedby asthma. In conclusion, TQ and CS was thought to be a promising treatment agent forasthma in the future especially during pregnancy. The use of TQ and CS in people withnormal asthma may be used as a new promising supplement to eliminate the negative effects of asthma

Keywords

• rats
• asthma
• remodeling
• vegf
• nebulized ovalbumin
• thymoquinone
• ceratonia siliqua.