Study of establishing disease-syndrome combined with animal model for immune thrombocytopenic purpura without additional conditions

Abstract

Objective: To explore the feasibility of establishing the disease-syndrome combined animal model for immune thrombocytopenic purpura (ITP) without additional conditions. Methods: Three batches of data related to the ITP model mice obtained by replication at different time were analyzed, and whether the APS-injected model mice replicated through the passive immune modeling method could simulate the pathogenesis and clinical characteristics of human ITP was evaluated according to the differentiation criteria for disease-syndrome combined model. Results: The APS-injected replicated ITP model mice possessed the following traits: (1) Compared with the normal group, the platelet count was significantly decreased, and coagulation time was significantly increased in the model group (P < .01). (2) Compared with the normal group, the medullary thrombocytogenous megakaryocytes were significantly decreased (P < .05, .01, .001). (3) The APS-injected sites and other parts of the model mice had spontaneous hemorrhage. (4) Behavioral changing signs were observed 1 week after the modeling (i.e. low activity, delayed activity, poor appetite, skin petechia/hemorrhage and spontaneous hemorrhage at the injected sites or other parts), and were getting more and more severe. Conclusion: According to the syndrome differentiation criteria for disease-syndrome combined model of ITP, the APS-injected animal model of ITP replicated through the passive immune modeling method without additional conditions possesses the characteristics of disease-syndrome combined model. It provides an ideal tool for the development of traditional Chinese medicine pharmacology experiment.

Keywords

• Immune thrombocytopenic purpura
• Syndrome of failure of spleen qi to control blood due to deficiency of spleen qi
• Disease-syndrome combined animal model