Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, United States; Ludwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, United States
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, United States; Ludwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, United States
Victoria L Mascetti
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, United States; Ludwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, United States
Janos Demeter
Baxter Laboratory, Department of Microbiology and Immunology and Department of Pathology, Stanford University School of Medicine, Stanford, United States
Benson George
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, United States; Ludwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, United States
Monika Zukowska
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, United States; Ludwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, United States
Gunsagar S Gulati
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, United States; Ludwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, United States
Rahul Sinha
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, United States; Ludwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, United States
Ryan A Flynn
Department of Chemistry, Stanford University, Stanford, United States
Allison Banuelos
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, United States; Ludwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, United States
Allison Zhang
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, United States; Ludwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, United States
Adam C Wilkinson
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, United States
Peter Jackson
Baxter Laboratory, Department of Microbiology and Immunology and Department of Pathology, Stanford University School of Medicine, Stanford, United States
Irving L Weissman
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, United States; Ludwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, United States; Department of Developmental Biology and the Stanford UC-Berkeley Stem Cell Institute, Stanford, United States; Department of Pathology, Stanford University School of Medicine, Stanford, United States
The balance of hematopoietic stem cell (HSC) self-renewal and differentiation is critical for a healthy blood supply; imbalances underlie hematological diseases. The importance of HSCs and their progenitors have led to their extensive characterization at genomic and transcriptomic levels. However, the proteomics of hematopoiesis remains incompletely understood. Here we report a proteomics resource from mass spectrometry of mouse young adult and old adult mouse HSCs, multipotent progenitors and oligopotent progenitors; 12 cell types in total. We validated differential protein levels, including confirmation that Dnmt3a protein levels are undetected in young adult mouse HSCs until forced into cycle. Additionally, through integrating proteomics and RNA-sequencing datasets, we identified a subset of genes with apparent post-transcriptional repression in young adult mouse HSCs. In summary, we report proteomic coverage of young and old mouse HSCs and progenitors, with broader implications for understanding mechanisms for stem cell maintenance, niche interactions and fate determination.