Проблемы особо опасных инфекций (Jun 2010)

The Influence of Growth Conditions of <i>Yersinia pestis</i> EV NIIEG Strain on Its Adhesion Properties

  • V. A. Oborin,
  • E. V. Pimenov,
  • A. G. Ivonin

DOI
https://doi.org/10.21055/0370-1069-2010-3(105)-54-57
Journal volume & issue
Vol. 0, no. 3(105)
pp. 54 – 57

Abstract

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Adhesion properties of vaccine Y. pestis EV NIIEG strain cells, cultivated in different conditions, were identified by photocolorimetric method. It was elucidated that the temperature of cultivation and the composition of nutrient media influence essentially the formation of surface structures of bacteria responsible for attachment to human red blood cells. The highest level of adhesion was demonstrated by the microbial cells cultivated at (28±1) °C on GRM-agar and Hottinger agar. Liophilisation of Y. pestis EV NIIEG cell as well as washing off extracellular metabolites did not affect the adhesion property of bacteria. The results received enabled to recommend the photocolorimetric method for study of adhesion activity of microbial cultures which were the basis of live vaccines.

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