Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus)

Weijie Chen; Jianchang Huang; Wei Wang; Ying Wang; Hao Chen; Qiyao Wang; Yuanxing Zhang; Qin Liu; Dahai Yang

Fundamental Research (Jul 2022)

Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus)

Weijie Chen,
Jianchang Huang,
Wei Wang,
Ying Wang,
Hao Chen,
Qiyao Wang,
Yuanxing Zhang,
Qin Liu,
Dahai Yang

Affiliations

Weijie Chen: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
Jianchang Huang: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
Wei Wang: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
Ying Wang: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
Hao Chen: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
Qiyao Wang: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
Yuanxing Zhang: Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China
Qin Liu: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China; Corresponding authors.
Dahai Yang: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China; Corresponding authors.

Journal volume & issue: Vol. 2, no. 4
pp. 550 – 561

Abstract

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In vertebrates, bony fishes possess not only innate immune cells but also T and B cells that are equivalent to those in mammals. However, the precise sub-cluster of immune cells in teleost fish remains largely unknown. Herein, we developed a dynamic bacterial infection model in turbot (Scophthalmus maximus) and created a fish immune cell landscape (FICL) for a primary lymphoid organ (head kidney), a secondary lymphoid organ (spleen), and barrier tissues (gills and posterior intestine). Moreover, through comprehensive characterization of the expression profiles of 16 clusters, including dendritic cells-like (DCs-like), macrophages (MΦs), neutrophils, NK cells, as well as 12 sub-clusters of T and B cells, we found that CD8+ CTLs, CD4−CD8− T, Th17 and ILC3-2 like cells possess a bifunctional role associated with cytotoxicity and immunoregulation during bacterial infection. To our knowledge, these results could provide a useful resource for a better understanding of immune cells in teleost fish and could act as a comprehensive knowledge base for assessing the evolutionary mechanism of adaptive immunity in vertebrates.

Published in Fundamental Research

ISSN: 2667-3258 (Online)
Publisher: KeAi Communications Co. Ltd.
Country of publisher: China
LCC subjects: Science: Science (General)
Website: https://www.keaipublishing.com/en/journals/fundamental-research/

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