Real-time RT-PCR Detection of HCN4 and ADAM8 genes in ventilator-associated pneumonia patients Hospitalized in intensive care unit

Abstract

IntroductionVentilator-associated pneumonia (VAP) is One of the most serious and prevalence of complication among patients in the intensive care unit nosocomial infections, which are often recognized after detecting symptoms. To date, there has been no proper clinical and diagnostic marker for early detection of this disease. In this study, two HCN4 and ADAM8 genes in patients with VAP were assessed to be used as a biomarker to recognize and distinguish the disease. MethodologyThis study was done in Masih Daneshvari Hospital affiliated to Shahid Beheshti University of Medical Sciences,Tehran,Iran since 2015-2016 and was case /control study . current study was administered by using peripheral blood samples, including 30 patients with VAP and 30 Healthy person. First, the peripheral blood samples were taken and then RNA was extracted and then synthesis CDNA. Finally, the evaluation of genes was performed by Realtime PCR method. FindingsIn peripheral blood samples, patients individuals , 10 out of 30 cases had positive HCN4 markers. Among the healthy individuals, 6 out of 30 cases had positive HCN4 biomarkers. Moreover, in ADAM8 marker patients individuals, 13 out of 30 cases had positive ADAM8 marker and 8 out of 30 healthy individuals had positive ADAM8 biomarkers.ConclusionGenes HCN4 and ADAM8 assessment with Real Time-PCR in this study can be used as promising markers in early detection of VAP disease. More extensive studies on larger sample sizes may yield higher sensitivity for these molecular markers.

Keywords

• Real-time RT-PCR, HCN4, ADAM8 genes, pneumonia, intensive care