Amelioration of oxidative stress, inflammation and tumor promotion by Tin oxide-Sodium alginate-Polyethylene glycol-Allyl isothiocyanate nanocomposites on the 1,2-Dimethylhydrazine induced colon carcinogenesis in rats

Abstract

Background: Colorectal cancer (CRC) is a frequently diagnosed cancer that primarily affects the digestive system and an imperative cause of mortalities worldwide. Objective: In this work, we formulated the Tin oxide-Sodium alginate-Polyethylene glycol-Allyl isothiocyanate nanocomposites (SAP-Ally-NCs) and investigated its anticancer role against the DMH-provoked CRC in rats. Methodology: The formulated SAP-Ally-NCs were characterized different techniques. The CRC was provoked to the rats via injecting 20 mg/kg of DMH and then administered with the formulated SAP-Ally-NCs for 16 weeks. The bodyweight changes and the polyp’s incidences were detected and tabulated. The status of lipid peroxidation and antioxidant enzymes were studied by standard techniques. The inflammatory markers and xenobiotic enzymes level was scrutinized using respective kits. The mRNA expressions of various signaling molecules were examined by RT-PCR. The liver and colon tissues were examined microscopically to detect the histological changes. Results: The formulated SAP-Ally-NCs treatment appreciably improved the body weight gain and suppressed the polyp’s incidences in the DMH-challenged animals. SAP-Ally-NCs treated animals were demonstrated the notable reduction in the lipid peroxidation and inflammatory cytokines and elevated the antioxidant enzymes i.e. CAT and SOD activity. SAP-Ally-NCs administered animals exhibited the noticeable reduction in the expression of PCNA, cyclin-D1, iNOS, and COX-2 in the colon tissues. The histological findings also unveiled the therapeutic role of SAP-Ally-NCs. Conclusion: In conclusion, the SAP-Ally-NCs demonstrated the potent anticancer action against the DMH-provoked CRC in rats. In future, it could be a potent chemotherapeutic agent to the CRC.

Keywords

• Colon cancer
• Oxidative stress
• Inflammation
• Allyl isothiocyanate
• 1,2-Dimethyl hydrazine