Communications Biology (Jun 2021)
LINE-1 transcription in round spermatids is associated with accretion of 5-carboxylcytosine in their open reading frames
- Martin J. Blythe,
- Ayhan Kocer,
- Alejandro Rubio-Roldan,
- Tom Giles,
- Abdulkadir Abakir,
- Côme Ialy-Radio,
- Lee M. Wheldon,
- Oxana Bereshchenko,
- Stefano Bruscoli,
- Alexander Kondrashov,
- Joël R. Drevet,
- Richard D. Emes,
- Andrew D. Johnson,
- John R. McCarrey,
- Daniel Gackowski,
- Ryszard Olinski,
- Julie Cocquet,
- Jose L. Garcia-Perez,
- Alexey Ruzov
Affiliations
- Martin J. Blythe
- Deep Seq, University of Nottingham, Queen’s Medical Centre
- Ayhan Kocer
- GReD Laboratory, CNRS UMR 6293 - INSERM U1103 - Clermont Université
- Alejandro Rubio-Roldan
- GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada
- Tom Giles
- Digital Research Service, Sutton Bonington Campus, University of Nottingham
- Abdulkadir Abakir
- School of Medicine, University of Nottingham, University Park
- Côme Ialy-Radio
- INSERM U1016, Institut Cochin - CNRS UMR8104 - Faculté de Médecine, Université Paris Descartes, Sorbonne Paris Cité
- Lee M. Wheldon
- Medical Molecular Sciences, University of Nottingham, University Park
- Oxana Bereshchenko
- Department of Medicine, Section of Pharmacology, University of Perugia
- Stefano Bruscoli
- Department of Medicine, Section of Pharmacology, University of Perugia
- Alexander Kondrashov
- School of Medicine, University of Nottingham, University Park
- Joël R. Drevet
- GReD Laboratory, CNRS UMR 6293 - INSERM U1103 - Clermont Université
- Richard D. Emes
- Digital Research Service, Sutton Bonington Campus, University of Nottingham
- Andrew D. Johnson
- School of Life Sciences, University of Nottingham, University Park
- John R. McCarrey
- University of Texas at San Antonio
- Daniel Gackowski
- Department of Clinical Biochemistry, Collegium Medicum, Nicolaus Copernicus University
- Ryszard Olinski
- Department of Clinical Biochemistry, Collegium Medicum, Nicolaus Copernicus University
- Julie Cocquet
- INSERM U1016, Institut Cochin - CNRS UMR8104 - Faculté de Médecine, Université Paris Descartes, Sorbonne Paris Cité
- Jose L. Garcia-Perez
- GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada
- Alexey Ruzov
- School of Medicine, University of Nottingham, University Park
- DOI
- https://doi.org/10.1038/s42003-021-02217-8
- Journal volume & issue
-
Vol. 4,
no. 1
pp. 1 – 11
Abstract
Blythe et al investigate the patterns of oxidized forms of the 5-methylcytosine DNA modification during spermatogenesis. They find that open reading frames of evolutionarily young and transcriptionally active LINE-1 retrotransposons are enriched in 5-carboxylcytosine (5caC) during spermatid maturation, suggesting a link between 5cac and LINE-1 activity.
