Instillation of Particulate Suspensions to the Lungs
Etsushi Kuroda,
Yasuo Morimoto,
Ken Ishii
Affiliations
Etsushi Kuroda
Laboratory of Adjuvant Innovation, Center for Vaccine and Adjuvant Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, JapanLaboratory of Vaccine Science, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
Yasuo Morimoto
Department of Occupational Pneumology, University of Occupational and Environmental Health, Japan, Fukuoka, Japan
Ken Ishii
Laboratory of Adjuvant Innovation, Center for Vaccine and Adjuvant Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, JapanLaboratory of Vaccine Science, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
Inhaled fine particulates are thought to cause chronic pulmonary inflammation through the deposition of particulates into the lungs. To investigate the effect of fine particulates on the lungs, instillation of suspension of particulates into the lungs is required. This protocol describes direct injection of suspensions of fine particulates into the airway. We also show examples of typical lung immune responses after particulate administration.
