Nature Communications (Jun 2019)
A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS)
- Ming-Ru Wu,
- Lior Nissim,
- Doron Stupp,
- Erez Pery,
- Adina Binder-Nissim,
- Karen Weisinger,
- Casper Enghuus,
- Sebastian R. Palacios,
- Melissa Humphrey,
- Zhizhuo Zhang,
- Eva Maria Novoa,
- Manolis Kellis,
- Ron Weiss,
- Samuel D. Rabkin,
- Yuval Tabach,
- Timothy K. Lu
Affiliations
- Ming-Ru Wu
- Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology
- Lior Nissim
- Department of Biochemistry and Molecular Biology, The Institute for Medical Research Israel-Canada, Hadassah Medical School, The Hebrew University of Jerusalem
- Doron Stupp
- Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, Hadassah Medical School, The Hebrew University of Jerusalem
- Erez Pery
- Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology
- Adina Binder-Nissim
- Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology
- Karen Weisinger
- Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology
- Casper Enghuus
- Department of Biological Engineering, Massachusetts Institute of Technology
- Sebastian R. Palacios
- Synthetic Biology Center, Massachusetts Institute of Technology
- Melissa Humphrey
- Brain Tumor Research Center, Department of Neurosurgery, Massachusetts General Hospital
- Zhizhuo Zhang
- Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology
- Eva Maria Novoa
- Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology
- Manolis Kellis
- Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology
- Ron Weiss
- Department of Biological Engineering, Massachusetts Institute of Technology
- Samuel D. Rabkin
- Brain Tumor Research Center, Department of Neurosurgery, Massachusetts General Hospital
- Yuval Tabach
- Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, Hadassah Medical School, The Hebrew University of Jerusalem
- Timothy K. Lu
- Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology
- DOI
- https://doi.org/10.1038/s41467-019-10912-8
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 10
Abstract
Synthetic promoters can be superior to native ones but the design is challenging without knowledge of gene regulation. Here the authors develop a pipeline that allows for screening a synthetic promoter library to identify high performance promoters in potentially any given cell state of interest.
