Data in Brief
(Oct 2018)
Prolonged DNA hydrolysis in water: A study on DNA stability
Paolo Fattorini,
Giorgio Marrubini,
Serena Bonin,
Barbara Bertoglio,
Pierangela Grignani,
Elisa Recchia,
Paola Pitacco,
Francesca Procopio,
Carolina Cantoni,
Irena Zupanič Pajnič,
Solange Sorçaburu-Cigliero,
Carlo Previdere`
Affiliations
Paolo Fattorini
Department of Medicine, Surgery and Health, University of Trieste, Italy; Corresponding author. Fax: +39 040 3996265.
Giorgio Marrubini
Department of Drug Sciences, University of Pavia, Italy
Serena Bonin
Department of Medicine, Surgery and Health, University of Trieste, Italy
Barbara Bertoglio
Department of Public Health, Experimental and Forensic Medicine, Section of Legal Medicine and Forensic Sciences, University of Pavia, Italy
Pierangela Grignani
Department of Public Health, Experimental and Forensic Medicine, Section of Legal Medicine and Forensic Sciences, University of Pavia, Italy
Elisa Recchia
Department of Medicine, Surgery and Health, University of Trieste, Italy
Paola Pitacco
Department of Medicine, Surgery and Health, University of Trieste, Italy
Francesca Procopio
School of Biological and Chemical Sciences, Queen Mary University of London, United Kingdom
Carolina Cantoni
CNR-ISMAR, Trieste, Italy
Irena Zupanič Pajnič
Institute of Forensic Medicine, Faculty of Medicine, University of Ljubljana, Slovenia
Solange Sorçaburu-Cigliero
Department of Medicine, Surgery and Health, University of Trieste, Italy
Carlo Previdere`
Department of Public Health, Experimental and Forensic Medicine, Section of Legal Medicine and Forensic Sciences, University of Pavia, Italy
Journal volume & issue
Vol. 20
Abstract
Read online
This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0–10 h in a previous study. Keywords: qPCR, Degraded samples, DNA quantification, DNA hydrolysis
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