A Comparison of Variations of Oxidative Stress Biomarkers in Patients with Primary Closed Angle Glaucoma and Healthy Subjects

Abstract

Background and Objectives: The importance of oxidative stress has been known in the formation and the pathogenesis of glaucoma. The alterations of serum oxidant/antioxidant profile in ocular pathologies, has been also stated in reports. This study was conducted to compare the oxidative stress biomarkers and antioxidant profile in patients with primary closed angle glaucoma (PCAG) and healthy individuals. Methods: This analytical case-control study was performed on 56 patients with PCAG (26 men and 30 women) referred to Ophthalmology department of Farabi and Hazrat Rasoul Hospitals and 80 healthy subjects (37 men and 43 women) as the control group. The concentration of malonyl dialdehyde (MDA) in red blood cells, total antioxidant capacity (TAC), reduced glutathione (GLT), and advanced oxidation protein products (AOPP) were determined. Data were analyzed using independent t- and chi-square tests. The significance level was considered to be p≤0.05. Results: The concentration of hemolysate MDA significantly increased in the patients with PCAG compared to healthy subjects (p=0.015), while the serum levels of TAC showed a significant decrease in these patients (p=0.016). Also, the serum level of AOPP levels significantly increased in patients with primary closed angle glaucoma compared to healthy subjects (p<0.019). Conclusion: The results of this study showed that increased lipid peroxidation and biological protein oxidation and also a significant decrease in total antioxidant capacity in PCAG patients compared to healthy subjects are indicative of the pathological role of increased oxidative damage in PCAG.

Keywords

• glaucoma
• angle-closure
• oxidative stress
• antioxidants