In vitro regeneration of Pistacia vera L. from nodal explants

Abstract

To enhance the induction of shoots, the excised nodes were cultured on Murashige and Skoog medium containing different cytokinins and various concentrations. The best conditions for shoot induction and growth were with 6-benzyladenine at 1.0 mg.l-1. For axillary shoot proliferation, the excellent result was obtained using 2.0 mg.l-1 meta-topoline. Well-developed shoots (more than 2 cm in length) were successfully rooted ex vitro at 82% by treatment with commercial rooting powder (2% indole-3-butyric acid; Rhizopon®). Rooted plantlets were acclimatized in the greenhouse and the survival rate of transplantation reached 80%.

Keywords

• sterilizing agents
• morphogenic response
• cytokinin
• shoot proliferation
• ex vitro rooting
• tissue culture