Engineered transfer RNAs for suppression of premature termination codons

John D. Lueck; Jae Seok Yoon; Alfredo Perales-Puchalt; Adam L. Mackey; Daniel T. Infield; Mark A. Behlke; Marshall R. Pope; David B. Weiner; William R. Skach; Paul B. McCray; Christopher A. Ahern

doi:10.1038/s41467-019-08329-4

Nature Communications (Feb 2019)

Engineered transfer RNAs for suppression of premature termination codons

John D. Lueck,
Jae Seok Yoon,
Alfredo Perales-Puchalt,
Adam L. Mackey,
Daniel T. Infield,
Mark A. Behlke,
Marshall R. Pope,
David B. Weiner,
William R. Skach,
Paul B. McCray,
Christopher A. Ahern

Affiliations

John D. Lueck: Department of Physiology and Pharmacology, University of Rochester School of Medicine and Dentistry
Jae Seok Yoon: CFFT Lab, Cystic Fibrosis Foundation Therapeutics
Alfredo Perales-Puchalt: The Wistar Institute
Adam L. Mackey: Department of Molecular Physiology and Biophysics, Iowa Neuroscience Institute, Carver College of Medicine, University of Iowa
Daniel T. Infield: Department of Molecular Physiology and Biophysics, Iowa Neuroscience Institute, Carver College of Medicine, University of Iowa
Mark A. Behlke: Integrated DNA Technologies Inc.
Marshall R. Pope: Department of Molecular Physiology and Biophysics, Iowa Neuroscience Institute, Carver College of Medicine, University of Iowa
David B. Weiner: The Wistar Institute
William R. Skach: CFFT Lab, Cystic Fibrosis Foundation Therapeutics
Paul B. McCray: Stead Family Department of Pediatrics, Pappajohn Biomedical Institute, University of Iowa
Christopher A. Ahern: Department of Molecular Physiology and Biophysics, Iowa Neuroscience Institute, Carver College of Medicine, University of Iowa

DOI: https://doi.org/10.1038/s41467-019-08329-4
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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Premature termination codon suppression therapy could be used to treat a range of genetic disorders. Here the authors present a high-throughput cell-based assay to identify anticodon engineered tRNAs with high suppression activity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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