Buffalo bbu-miR-493-5p Promotes Myoblast Proliferation and Differentiation

Liyin Zhang; Dandan Zhong; Chengxuan Yao; Qingyou Liu; Deshun Shi; Mingsheng Jiang; Jian Wang; Zhaocheng Xiong; Hui Li

doi:10.3390/ani14040533

Animals (Feb 2024)

Buffalo bbu-miR-493-5p Promotes Myoblast Proliferation and Differentiation

Liyin Zhang,
Dandan Zhong,
Chengxuan Yao,
Qingyou Liu,
Deshun Shi,
Mingsheng Jiang,
Jian Wang,
Zhaocheng Xiong,
Hui Li

Affiliations

Liyin Zhang: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Dandan Zhong: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Chengxuan Yao: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Qingyou Liu: Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528225, China
Deshun Shi: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Mingsheng Jiang: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Jian Wang: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Zhaocheng Xiong: Research & Development Affairs Office, Guangxi University, Nanning 530004, China
Hui Li: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning 530004, China

DOI: https://doi.org/10.3390/ani14040533
Journal volume & issue: Vol. 14, no. 4
p. 533

Abstract

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In recent years, the meat and dairy value of buffaloes has become a major concern in buffalo breeding, and the improvement of buffalo beef quality is key to protecting buffalo germplasm resources and solving the problem of beef supply. MiRNAs play a significant role in regulating muscle development. However, the precise mechanism by which they regulate the development of buffalo skeletal muscles remains largely unexplored. In this study, we examined miRNA expression profiles in buffalo myoblasts during the proliferation and differentiation stages. A total of 177 differentially expressed miRNAs were identified, out of which 88 were up-regulated and 89 down-regulated. We focused on a novel miRNA, named bbu-miR-493-5p, that was significantly differentially expressed during the proliferation and differentiation of buffalo myoblasts and highly expressed in muscle tissues. The RNA-FISH results showed that bbu-miR-493-5p was primarily located in the cytoplasm to encourage buffalo myoblasts’ proliferation and differentiation. In conclusion, our study lays the groundwork for future research into the regulatory role of miRNAs in the growth of buffalo muscle.

Published in Animals

ISSN: 2076-2615 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture: Animal culture: Veterinary medicine; Science: Zoology
Website: http://www.mdpi.com/journal/animals/

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