Ukrainian Scientific Medical Youth Journal (Apr 2016)
INDICATORS CELL CYCLE IN ADENOHYPOPHYSIS A LATER DATE AFTER BURN INJURY IN RATS FOR SKIN CONDITIONS SEPARATE INFUSION IN THE FIRST 7 DAYS OF 0,9% NACL SOLUTION, SOLUTIONS LACTOPROTEIN WITH SORBITOL OR HAES-LX-5%
Abstract
The paper is aimed at the comparative analysis of the rates of rat adenohypophysis cell cycle and DNA- fragmentation following the 14, 21 i 30 days after thermal burn in rehabilitation with 0,9 % NaCl solution, lactoprotein with sorbitol and HAES-LX-5%. Materials and Methods. The experimental study has been made on 100 Wistar white male rats, weighted 160- ISO g, provided by the vivarium at the Institute of Pharmacology and Toxicology of NAMS of Ukraine, and carried out on the basis of the Research Laboratory of Functional Morphology and Genetics of Research Center at National Pirogov Memorial Medical University, Vinnytsya. Before simulation of pathological state the lateral surfaces of animals' bodies were shaved with shaver and safety razor. The infusion of 0.9% NaCl solution, lactoprotein with sorbitol or HAES-LX-5% was conducted in the lower hollow vein after its catheterization through the femoral vein, made in aseptic conditions. Catheter was sewed subcutaneously, its lumen was filled with heparin titrating solution (0,1 ml heparin per 10 ml 0.9% NaCl solution) after each administration of agents. Infusions were performed once a day during the first 7 days. Catheterization of great vessels and animals decapitation were made under 60 mg/kg intravenous propofol anesthesia. Flow analysis was made on multifunctional research flow cytometer “Partec PAS” (Partec, Germany) in the Research Center at N.I. Pirogov Vinnytsya National Medical University. The analysis of cell cycle was made by means of FloMax (Partec, Germany) software in full digital compliance with mathematical model. Statistical processing of the results was carried out in the STATISTICA 6.1 license package using the nonparametric methods of the evaluation of the obtained results. Results. Apply within 7 days 0.9% solution of NaCl, Lactoproteinum with sorbitol or HAES-LX 5% does not affect the performance of the cell cycle and DNA fragmentation adenohypophysis cells intact rats. In the context of thermal damage to the skin and using 0.9% NaCl solution adenohypophysis cells 14 days after the skin care values of S-phase at 115.6% (p <0.01) than in the control group animals. The value of S-phase indicator reaches a maximum of 21 days (at 205.3%, p <0,01 bilsha than in the control group), and after 30 days the figure is reduced but remains at 96.2% (p <0.01) higher than the level recorded in the control group rats.
