MORPHO-FUNCTIONAL CHARACTERISTIC OF ENTEROBACTERIA ADHESIVE ACTIVITY

Abstract

Aim. Study adhesive activity of enterobacteria in the model of hemagglutination reaction with animal and avian erythrocytes and clarify structures responsible for adhesion in enterobacteria. Materials and methods. 58 cultures ofenterobacteria were used, ofwhich: 21 Escherichia coli strains, 13 Citrobacter spp., 11 Morganella morganii, 9 Proteus spp., 4 Hafnia alvei. Erythrocytes of various animals and birds were used in hemagglutination reaction. Electron-microscopical studies were carried out in JEM-100B (Japan) electron microscope. Results. Use of avian erythrocytes as a target of adhesion determination, compared with animal erythrocytes, has shown that bacteria can cause D-mannose-sensitive hemagglutination reaction, linked with the presence of110 - 420 nm long and 5.0 - 5.4 nm wide cilia in the microorganisms. Conclusion. Adhesion of enterobacteria was shown to be a complex process, depending on the presence of certain fimbrial structures, use of those results in specific interaction of the microbe with certain host cell receptors. Avian erythrocytes are a model target cells

Keywords

• enterobacteria
• adhesive activity
• fimbria
• pili