Exploring the Induction Mechanism of Chitosan Oligosaccharide on the Cold Resistance of Rice Seedlings by Proteomic Technology

Abstract

Rice seedlings are highly sensitive to low temperature. Therefore it is imperative to apply new biopesticides, e.g. chitosan oligosaccharides in rice production to help rice resist low temperature stress. However, the mechanism of chitosan oligosaccharides inducing rice resistance against low temperature stress has remained unclear. In this study, Minghui 63 rice was selected to simulate the whole process of rice seedling from normal growth to low temperature stress and then to regeneration. The physiological and biochemical indexes of rice seedlings in the corresponding periods were determined, and the metabolic pathways of differential proteins of rice seedlings were identified and analyzed by label-free quantitative proteomics technology, so as to explore the induction mechanism of chitosan oligosaccharides on rice seedlings resistance to low temperature stress. The photosynthetic pathway and starch sucrose metabolism were also discussed in detail. The results showed that chitosan oligosaccharides could regulate the abundance of common proteins in rice and resist the influence of low temperature stress on photosynthesis. Chitosan oligosaccharides also had a certain impact on the abundance of expressed proteins. It could also promote sucrose decomposition and starch accumulation to convert into glucose donors for rice growth and metabolism. This study provides important practical guidance for the breeding of cold-resistant rice, and scientific basis for the research and utilization of cold-resistant rice and new biological pesticides.

Keywords

• rice seedlings
• low temperature stress
• physiology and biochemistry
• proteomics