Frontiers in Microbiology (Dec 2020)
A Cross-Protective Vaccine Against 4b and 1/2b Listeria monocytogenes
- Fanzeng Meng,
- Fanzeng Meng,
- Fanzeng Meng,
- Fanzeng Meng,
- Tengfei Zhu,
- Tengfei Zhu,
- Tengfei Zhu,
- Tengfei Zhu,
- Hao Yao,
- Hao Yao,
- Hao Yao,
- Hao Yao,
- Zhiting Ling,
- Zhiting Ling,
- Zhiting Ling,
- Zhiting Ling,
- Youwei Feng,
- Youwei Feng,
- Youwei Feng,
- Youwei Feng,
- Guo Li,
- Guo Li,
- Guo Li,
- Guo Li,
- Jing Li,
- Jing Li,
- Jing Li,
- Jing Li,
- Xinyu Sun,
- Xinyu Sun,
- Xinyu Sun,
- Xinyu Sun,
- Jiaqi Chen,
- Jiaqi Chen,
- Jiaqi Chen,
- Jiaqi Chen,
- Chuang Meng,
- Chuang Meng,
- Chuang Meng,
- Chuang Meng,
- Xin’an Jiao,
- Xin’an Jiao,
- Xin’an Jiao,
- Xin’an Jiao,
- Yuelan Yin,
- Yuelan Yin,
- Yuelan Yin,
- Yuelan Yin
Affiliations
- Fanzeng Meng
- Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
- Fanzeng Meng
- Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, The Ministry of Agriculture of China, Yangzhou University, Yangzhou, China
- Fanzeng Meng
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Fanzeng Meng
- Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, China
- Tengfei Zhu
- Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
- Tengfei Zhu
- Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, The Ministry of Agriculture of China, Yangzhou University, Yangzhou, China
- Tengfei Zhu
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Tengfei Zhu
- Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, China
- Hao Yao
- Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
- Hao Yao
- Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, The Ministry of Agriculture of China, Yangzhou University, Yangzhou, China
- Hao Yao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Hao Yao
- Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, China
- Zhiting Ling
- Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
- Zhiting Ling
- Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, The Ministry of Agriculture of China, Yangzhou University, Yangzhou, China
- Zhiting Ling
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Zhiting Ling
- Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, China
- Youwei Feng
- Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
- Youwei Feng
- Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, The Ministry of Agriculture of China, Yangzhou University, Yangzhou, China
- Youwei Feng
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Youwei Feng
- Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, China
- Guo Li
- Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
- Guo Li
- Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, The Ministry of Agriculture of China, Yangzhou University, Yangzhou, China
- Guo Li
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Guo Li
- Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, China
- Jing Li
- Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
- Jing Li
- Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, The Ministry of Agriculture of China, Yangzhou University, Yangzhou, China
- Jing Li
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Jing Li
- Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, China
- Xinyu Sun
- Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
- Xinyu Sun
- Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, The Ministry of Agriculture of China, Yangzhou University, Yangzhou, China
- Xinyu Sun
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Xinyu Sun
- Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, China
- Jiaqi Chen
- Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
- Jiaqi Chen
- Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, The Ministry of Agriculture of China, Yangzhou University, Yangzhou, China
- Jiaqi Chen
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Jiaqi Chen
- Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, China
- Chuang Meng
- Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
- Chuang Meng
- Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, The Ministry of Agriculture of China, Yangzhou University, Yangzhou, China
- Chuang Meng
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Chuang Meng
- Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, China
- Xin’an Jiao
- Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
- Xin’an Jiao
- Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, The Ministry of Agriculture of China, Yangzhou University, Yangzhou, China
- Xin’an Jiao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Xin’an Jiao
- Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, China
- Yuelan Yin
- Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
- Yuelan Yin
- Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, The Ministry of Agriculture of China, Yangzhou University, Yangzhou, China
- Yuelan Yin
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Yuelan Yin
- Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, China
- DOI
- https://doi.org/10.3389/fmicb.2020.569544
- Journal volume & issue
-
Vol. 11
Abstract
Listeria monocytogenes (Lm) is a foodborne zoonotic pathogen that causes listeriosis with a mortality rate of 20–30%. Serovar 4b and 1/2b isolates account for most of listeriosis outbreaks, however, no listeriosis vaccine is available for either prophylactic or therapeutic use. Here, we developed a triple-virulence-genes deletion vaccine strain, and evaluated its safety, immunogenicity, and cross-protective efficiency. The virulence of NTSNΔactA/plcB/orfX was reduced 794-folds compared with the parental strain. Additionally, it was completely eliminated in mice at day 7 post infection and no obvious pathological changes were observed in the organs of mice after prime-boost immunization for 23 days. These results proved that the safety of the Lm vaccine strain remarkably increased. More importantly, the NTSNΔactA/plcB/orfX strain stimulated higher anti-Listeriolysin O (LLO) antibodies, induced significantly higher expression of IFN-γ, TNF-α, IL-17, and IL-6 than the control group, and afforded 100% protection against serovar 4b and 1/2b challenges. Taken together, our research demonstrates that the triple-genes-deletion vaccine has high safety, can elicit strong Th1 type immune response, and affords efficient cross-protection against two serovar Lm strains. It is a promising vaccine for prevention of listeriosis.
Keywords
- Listeria monocytogenes
- attenuated vaccine
- cellular immune response
- cross protection
- humoral immune response
