Frontiers in Plant Science (Aug 2023)
Integrative transcriptome and metabolome analysis reveals the mechanisms of light-induced pigmentation in purple waxy maize
- Yuan Lu,
- Yuan Lu,
- Yuan Lu,
- Yuan Lu,
- Yao Yu,
- Yao Yu,
- Yao Yu,
- Yao Yu,
- Yanfang Xuan,
- Ayiguli Kari,
- Ayiguli Kari,
- Ayiguli Kari,
- Ayiguli Kari,
- Caixia Yang,
- Caixia Yang,
- Caixia Yang,
- Caixia Yang,
- Chenyu Wang,
- Chenyu Wang,
- Chenyu Wang,
- Chenyu Wang,
- Chao Zhang,
- Chao Zhang,
- Chao Zhang,
- Chao Zhang,
- Wei Gu,
- Wei Gu,
- Wei Gu,
- Wei Gu,
- Hui Wang,
- Hui Wang,
- Hui Wang,
- Hui Wang,
- Yingxiong Hu,
- Yingxiong Hu,
- Yingxiong Hu,
- Yingxiong Hu,
- Pingdong Sun,
- Pingdong Sun,
- Pingdong Sun,
- Pingdong Sun,
- Yuan Guan,
- Yuan Guan,
- Yuan Guan,
- Yuan Guan,
- Wenshuai Si,
- Bing Bai,
- Xuecai Zhang,
- Yunbi Xu,
- Yunbi Xu,
- Yunbi Xu,
- Boddupalli M. Prasanna,
- Biao Shi,
- Hongjian Zheng,
- Hongjian Zheng,
- Hongjian Zheng,
- Hongjian Zheng
Affiliations
- Yuan Lu
- Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yuan Lu
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yuan Lu
- Shanghai Engineering Research Center of Specialty Maize, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yuan Lu
- Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, China
- Yao Yu
- Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yao Yu
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yao Yu
- Shanghai Engineering Research Center of Specialty Maize, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yao Yu
- Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, China
- Yanfang Xuan
- Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Ayiguli Kari
- Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Ayiguli Kari
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Ayiguli Kari
- Shanghai Engineering Research Center of Specialty Maize, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Ayiguli Kari
- Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, China
- Caixia Yang
- Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Caixia Yang
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Caixia Yang
- Shanghai Engineering Research Center of Specialty Maize, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Caixia Yang
- Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, China
- Chenyu Wang
- Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Chenyu Wang
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Chenyu Wang
- Shanghai Engineering Research Center of Specialty Maize, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Chenyu Wang
- Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, China
- Chao Zhang
- Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Chao Zhang
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Chao Zhang
- Shanghai Engineering Research Center of Specialty Maize, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Chao Zhang
- Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, China
- Wei Gu
- Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Wei Gu
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Wei Gu
- Shanghai Engineering Research Center of Specialty Maize, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Wei Gu
- Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, China
- Hui Wang
- Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Hui Wang
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Hui Wang
- Shanghai Engineering Research Center of Specialty Maize, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Hui Wang
- Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, China
- Yingxiong Hu
- Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yingxiong Hu
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yingxiong Hu
- Shanghai Engineering Research Center of Specialty Maize, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yingxiong Hu
- Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, China
- Pingdong Sun
- Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Pingdong Sun
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Pingdong Sun
- Shanghai Engineering Research Center of Specialty Maize, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Pingdong Sun
- Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, China
- Yuan Guan
- Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yuan Guan
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yuan Guan
- Shanghai Engineering Research Center of Specialty Maize, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yuan Guan
- Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, China
- Wenshuai Si
- Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Bing Bai
- Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Xuecai Zhang
- International Maize and Wheat Improvement Center (CIMMYT), Texcoco, Mexico
- Yunbi Xu
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Yunbi Xu
- International Maize and Wheat Improvement Center (CIMMYT), Texcoco, Mexico
- Yunbi Xu
- Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
- Boddupalli M. Prasanna
- International Maize and Wheat Improvement Center (CIMMYT), Nairobi, Kenya
- Biao Shi
- Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai, China
- Hongjian Zheng
- Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Hongjian Zheng
- CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Hongjian Zheng
- Shanghai Engineering Research Center of Specialty Maize, Shanghai Academy of Agricultural Sciences, Shanghai, China
- Hongjian Zheng
- Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, China
- DOI
- https://doi.org/10.3389/fpls.2023.1203284
- Journal volume & issue
-
Vol. 14
Abstract
IntroductionWaxy maize, mainly consumed at the immature stage, is a staple and vegetable food in Asia. The pigmentation in the kernel of purple waxy maize enhances its nutritional and market values. Light, a critical environmental factor, affects anthocyanin biosynthesis and results in pigmentation in different parts of plants, including in the kernel. SWL502 is a light-sensitive waxy maize inbred line with purple kernel color, but the regulatory mechanism of pigmentation in the kernel resulting in purple color is still unknown.MethodsIn this study, cyanidin, peonidin, and pelargonidin were identified as the main anthocyanin components in SWL502, evaluated by the ultra-performance liquid chromatography (UPLC) method. Investigation of pigment accumulation in the kernel of SWL502 was performed at 12, 17, and 22 days after pollination (DAP) under both dark and light treatment conditions via transcriptome and metabolome analyses.ResultsDark treatment affected genes and metabolites associated with metabolic pathways of amino acid, carbohydrate, lipid, and galactose, biosynthesis of phenylpropanoid and terpenoid backbone, and ABC transporters. The expression of anthocyanin biosynthesis genes, such as 4CL2, CHS, F3H, and UGT, was reduced under dark treatment. Dynamic changes were identified in genes and metabolites by time-series analysis. The genes and metabolites involved in photosynthesis and purine metabolism were altered in light treatment, and the expression of genes and metabolites associated with carotenoid biosynthesis, sphingolipid metabolism, MAPK signaling pathway, and plant hormone signal transduction pathway were induced by dark treatment. Light treatment increased the expression level of major transcription factors such as LRL1, myc7, bHLH125, PIF1, BH093, PIL5, MYBS1, and BH074 in purple waxy maize kernels, while dark treatment greatly promoted the expression level of transcription factors RVE6, MYB4, MY1R1, and MYB145.DiscussionThis study is the first report to investigate the effects of light on waxy maize kernel pigmentation and the underlying mechanism at both transcriptome and metabolome levels, and the results from this study are valuable for future research to better understand the effects of light on the regulation of plant growth.
Keywords
