CONSEQUENCES OF AD LIBITUM ALUMINIUM ADMINISTRATION DURING THREE GENERATIONS ON RATS BLOOD GSH DINAMICS

Abstract

The influence of ad libitum aluminium sulphate, Al2(SO4)3, administration during three generations on the blood glutathione (GSH) values in rats was studied. This study was carried out on 34 Wistar rats divided in 9 experimental batches (LI , LII, LIII, LIF1n, LIIF1n, LIIIF1n, LIF2n, LIIF2n, LIIIF2n )and one control batch (C). Aluminium sulphate has been administrated in drinking water, ad libitum. GSH was measured quantitatively at Perkin-Elmer spectrophotometer through Beutler et. al. method (4), at 412nm. Hemoglobin (Hb) was measured through Drabkin method (9) at the automatic analyzer Bekman Coulter. GSH is considered to be the most powerful and most important of body’s self-generated antioxidants. It is a powerful antioxidant, he neutralizes free radicals and prevents their formation. Because aluminium (Al) can couse oxidative damage on cellular biological processes by inhibiting GSH regeneration through the inhibition of NADPH supply in mitochondria, but only a little inhibitory effect on the GSH generation in cytosol (17), the consequences of ad libitum Al2(SO4)3 administration was a limited decrease of GSH values.

Keywords

• gsh
• aluminium
• rats