Dynamic Structure of Yeast Septin by Fast Fluctuation-Enhanced Structured Illumination Microscopy

Longfang Yao; Li Zhang; Liwen Chen; Xingyu Gong; Jiahui Zhong; Baoju Wang; Yiyan Fei; Lan Mi; Jiong Ma

doi:10.3390/microorganisms9112255

Microorganisms (Oct 2021)

Dynamic Structure of Yeast Septin by Fast Fluctuation-Enhanced Structured Illumination Microscopy

Longfang Yao,
Li Zhang,
Liwen Chen,
Xingyu Gong,
Jiahui Zhong,
Baoju Wang,
Yiyan Fei,
Lan Mi,
Jiong Ma

Affiliations

Longfang Yao: Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, School of Information Science and Technology, Fudan University, 220 Handan Road, Shanghai 200433, China
Li Zhang: Shanghai Engineering Research Center of Industrial Microorganisms, The Multiscale Research Institute of Complex Systems (MRICS), School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China
Liwen Chen: Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, School of Information Science and Technology, Fudan University, 220 Handan Road, Shanghai 200433, China
Xingyu Gong: Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, School of Information Science and Technology, Fudan University, 220 Handan Road, Shanghai 200433, China
Jiahui Zhong: Institute of Biomedical Engineering and Technology, Academy for Engineer and Technology, Fudan University, 220 Handan Road, Shanghai 200433, China
Baoju Wang: Centre for Optical and Electromagnetic Research, Guangdong Provincial Key Laboratory of Optical Information Materials and Technology, South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510006, China
Yiyan Fei: Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, School of Information Science and Technology, Fudan University, 220 Handan Road, Shanghai 200433, China
Lan Mi: Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, School of Information Science and Technology, Fudan University, 220 Handan Road, Shanghai 200433, China
Jiong Ma: Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, School of Information Science and Technology, Fudan University, 220 Handan Road, Shanghai 200433, China

DOI: https://doi.org/10.3390/microorganisms9112255
Journal volume & issue: Vol. 9, no. 11
p. 2255

Abstract

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When Saccharomyces cerevisiae divides, a structure composed of different septin proteins arranged according to a certain rule is formed at the cell division site. The structure undergoes multiple remodeling stages during the cell cycle, thus guiding the yeast cells to complete the entire division process. Although the higher-order structure of septins can be determined using electron microscopy, the septin’s dynamic processes are poorly understood because of limitations in living cell super-resolution imaging technology. Herein, we describe a high lateral resolution and temporal resolution technique, known as fast fluctuation-enhanced structured illumination microscopy (fFE-SIM), which more than doubles the SIM resolution at a frame rate of 38 Hz in living cells. This allows a highly dynamic and sparse septin structure to be observed in Saccharomyces cerevisiae.

Published in Microorganisms

ISSN: 2076-2607 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.mdpi.com/journal/microorganisms

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