ANALYSIS OF AMINO ACIDS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

Amino acids are the building blocks of proteins and are considered as the key precursors for the formation of hormones and low molecular weight nitrogenous substances with biological importance. Since the analysis of amino acids has been carried out for both qualitative and quantitative purposes with an aim to study their levels in the plasma concentration, the quantitative determination, in particular, also helps in the diagnosis of different diseases associated with their deficiency. This review article deals with the determination of amino acids by chromatographic methods which include ion-exchange chromatography (IEC), high performance liquid chromatography (HPLC), reverse phase-high performance liquid chromatography (RP-HPLC) and ultra-performance liquid chromatography (UPLC). The review will also give an idea for the preparation of samples, derivatization methods for the analysis of amino acids (direct and indirect methods) and separation of amino acids by high performance liquid chromatographic technique.

Keywords

• HPLC
• amino acids
• derivatzation
• plasma