Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul (Jul 1999)
Study of the analgesic effect of Pimpinella Anisun extract by Tail-Flick and Formalin test in mice
Abstract
Objective: In traditional medicine, some plants have been used as analgesic. The aim of this investigation was to evaluate the analgesic effect of “Pimpinella Anisun” that is used traditionally to relieve headache and spastic pain. Methods: The seuhleted and percolated extracts of Pimpinella Anisun, with different doses, were injected intraperitoneally (IP) to mice and the analgesic effect was determined by tail-flick and Formalin test. Findings: The results show that suxhulated extract with doses of (25, 50, 100 and 200 mg/kg), induce significant analgesia in comparison with control group (P<0.01). In tail-flick test, the maximum analgesia was induced with the dose of 50 mg/kg and reached high to a peak at 45th min. after injection (P<0.01). The extract with the dose of 50 mg/kg induced analgesia in Formalin test at a different time (P<0.01) than in 0-5 min. There is no difference between the analgesic effect of morphine and extract at 10-15 min. in Formalin test, but at the first and second 5 min, the analgesic effect of extract was smaller than morphine (P<0.01) and was greater at 15-20 and 20-55 min. (P<0.01). There is no difference between the analgesic effect of extract and ASA 300 mg/kg at the first 5 minutes and 25-30, but at the second 5 minutes, the effect of extract was smaller (P<0.01) but it was greater than ASA at other times (P<0.01). Pretreatment of animals with naloxone did not change the analgesic effect of extract in the Formalin and tail-flick. Conclusion: This finding indicates that the involvement of opioid receptors in the analgesic effect of extract was excluded. In this investigation the suitable analgesic effect was seen from the extract of Pimpinella Anisun and therefore more investigation in this filed is needed.
