Gap-Free Nuclear and Mitochondrial Genomes of Ustilaginoidea virens JS60-2, a Fungal Pathogen Causing Rice False Smut

Yin Wang; Lei Yang; Qun Yang; Jie Dong; Yufu Wang; Yuhang Duan; Weixiao Yin; Lu Zheng; Wenxian Sun; Jing Fan; Chao-Xi Luo; Guotian Li

doi:10.1094/MPMI-07-22-0158-A

Molecular Plant-Microbe Interactions (Dec 2022)

Gap-Free Nuclear and Mitochondrial Genomes of Ustilaginoidea virens JS60-2, a Fungal Pathogen Causing Rice False Smut

Yin Wang,
Lei Yang,
Qun Yang,
Jie Dong,
Yufu Wang,
Yuhang Duan,
Weixiao Yin,
Lu Zheng,
Wenxian Sun,
Jing Fan,
Chao-Xi Luo,
Guotian Li

Affiliations

Yin Wang: State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, the Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China
Lei Yang: State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, the Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China
Qun Yang: State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, the Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China
Jie Dong: State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, the Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China
Yufu Wang: Key Lab of Horticultural Plant Biology, Ministry of Education, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China
Yuhang Duan: State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, the Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China
Weixiao Yin: Key Lab of Horticultural Plant Biology, Ministry of Education, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China
Lu Zheng: State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, the Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China
Wenxian Sun: College of Plant Protection and the Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management, China Agricultural University, Beijing, 100193, China
Jing Fan: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
Chao-Xi Luo: Key Lab of Horticultural Plant Biology, Ministry of Education, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China
Guotian Li: State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, the Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China

DOI: https://doi.org/10.1094/MPMI-07-22-0158-A
Journal volume & issue: Vol. 35, no. 12
pp. 1120 – 1123

Abstract

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Rice false smut (RFS), caused by Ustilaginoidea virens, has become a major disease in recent years, and mycotoxins produced by U. virens often threaten food safety. To study fungal pathogenesis and identify potential targets for developing new fungicides, gap-free nuclear and complete mitochondrial genomes of U. virens JS60-2 were sequenced and assembled. Using the second and third generation sequencing data, we assembled a 38.02-Mb genome that consists of seven contigs with the contig N50 being 6.32-Mb. In total, 8,486 protein-coding genes were annotated in the genome, including 21 secondary metabolism gene clusters. We also assembled the complete mitochondrial genome, which is 102,498 bp, with 28% GC content. The JS60-2 genomes assembled in this study will facilitate research on U. virens and contribute to RFS control. [Graphic: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Published in Molecular Plant-Microbe Interactions

ISSN: 0894-0282 (Print); 1943-7706 (Online)
Publisher: The American Phytopathological Society
Country of publisher: United States
LCC subjects: Science: Microbiology; Science: Botany
Website: https://apsjournals.apsnet.org/journal/mpmi

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